Physiological characterization of the human vasopressin transgenic rats.

为了研究慢性高加压素血症遗传模型的生理学后果，将小鼠镍硫蛋白-人精氨酸加压素（AVP）基因导入大鼠生殖细胞系，制备了转基因大鼠。通过几代分析一个稳定的转基因家系。通过Southern印迹证明了人AVP基因的表达，并导致脑中AVP的量增加。从中枢神经系统分泌AYP导致血浆中激素浓度增加，尿中排泄量增加，与正常大鼠相比增加5至10倍。在肾脏中发现异位激素产生。转基因大鼠在基础条件下血浆钠水平没有显著降低，但24小时尿量减少。这些数据可能表明转基因大鼠肾脏的抗利尿活性增加。

To study the physiologic consequences of a genetic model of chronic hypervasopressinemia, transgenic rat was produced by induction of a mouse nietallothionein-human arginine vasopressin (AVP) gene into the germ line of rat. One stable transgenic pedigree was analyzed through several generations. Expression of the human AVP gene was demonstrated by Southern blotting and resulted in increased amounts of AVP in brains. Secretion of AYP from the central nervous system results in an increased concentration of the hormone in the plasma and in an increased excretion in the urine in amounts five to ten times compared to those of normal rats. Ectopic hormone production was found in the kidney. Transgenicrat showed no significant decrease in plasma Na level but decreased 24-hour urine volume under basal conditions. These data might suggest increased antidiuretic activity on kidney in transgenic rat.