Effects of occlusal force on homeostasis of microcirculation in periodontal ligament.

1. 我们已经开发了一种体内微血管床实验模型，该模型可以在毛细血管后小静脉上产生0.5至1秒间隔的轻度间歇性机械压迫刺激。这种刺激被认为是咀嚼时对牙周韧带的压迫。我们使用活体显微镜观察了机械压迫期间和之后毛细血管后小静脉中血管通透性反应的增加和白细胞运动的变化，并在第29届韩国正牙医师协会科学大会上发表了这些结果。我们还观察了衰老对微循环对这种机械刺激的反应的影响，并在第55届日本正畸学会年会上发表了结果。我们利用免疫组织化学技术对诱导这些反应的因素进行了进一步的研究。结果，我们能够检测到p -选择素的出现，一种粘附分子，在压缩区域周围的内皮细胞上。我们也在第22届日本微循环学会年会上报道了这些结果。

1. We have developed an in vivo microvascular bed experimental model which produces the stimulation on a post capillary venule with mild intermittent mechanical compressions of 0.5 to 1-second intervals. This type of stimulation is assumed to be the compressions of periodontal ligament during mastication.2. We have observed the increased vascular permeability reaction and the changes of leukocyte movement in a post-capillary venule during and after mechanical compression using an intra-vital microscopy, and presented these results at 29th Scientific Congress of Korean Association of Orthodontists. We have also observed the effects of aging on the responses of microcirculation to such mechanical stimulation, and presented the results at 55th Annual Meeting of Japan Orthodontic Society.3. We have made another experiment to clarify the factors inducing these responses by means of immunohistochemical technique. As a result, we were able to detect the appearance of P-selectin, a kind of adhesion molecules, on the endothelial cells around the compressed area. We have also reported these results at 22th Annual Meeting of Japanese Society for Microcirculation.