Development of Artificial haching in the wild silkmoth, Antheraea yamamai

本课题组组长研制出了天蚕咪唑类化合物人工孵化器。本课题对咪唑类化合物人工孵化及其质量法进行了研究，取得了以下重要成果：1）筛选出了多种咪唑类化合物的滞育抑制和孵化率。因此，KK-62更有效地保持较高的孵化率。在滞育前期仔鱼的肠道神经支配和内分泌细胞中，发现了许多肽类物质。2）合成了一种新的有效的解除滞育化合物1- {5-（4-乙基苯氧基）戊基} -咪唑。3)实现了KK-62的批量合成。4)用餐具漂白剂和次氯酸钠溶液去除蚕卵壳的方法简便易行，可用于大规模咪唑类化合物的人工孵化，为天蚕昆虫技术的发展提供了依据。

The primitive artifical hatching with imidazole compound of Antheraea yamamai have been developed by the head investigator. In this project, improved artificial hatching with imidazole compound and it's mass method were examined and the following important results were obtained.1) Many imidazole compounds were screened for diapsue brekdown and hatchability. As a result, KK-62 was more effective to keep higher hatchability. Many peptide-like materials were immunohistochemically observed in the gut innervation and endocrine cells of diapausing pre-larvae. KK-42-binding protein (about 60 kDa) was also isolated from microsome fraction of diapausing pre-larvae.2) As a new and effective compund for diapause breakdown, 1- {5- (4-Ethylphenoxy) pentyl} -imidazole was synthesized. 3) A mass method for the synthesis of KK-62 was achieved. 4) A simple method to remove egg shell with tableware bleach and sodium hypochlorite solution was very effective for a mass artificial hatching with imidazole compound.These results will be very usefule for the development of insect technology used in the wild silkmoth Antheraea yamamai.