Superoxide Dismutase Mimic and Angiotensin I-Converting Enzyme Inhibitory Activities of Extractives from Wood

为开发柳杉的新用途，研究了柳杉提取物的生物活性、超氧化物歧化酶（SOD）模拟物和血管紧张素转化酶（ACE）抑制活性。在用正己烷、氯仿、乙醇和热水连续提取的根、叶、心材、边材、内树皮和外树皮的24种提取物中，心材和外树皮的乙醇提取物分别表现出最高的SOD模拟活性和ACE抑制活性。结果表明，从心材中分离鉴定出具有SOD活性的Sequrin-C，从外皮中分离鉴定出具有ACE抑制活性的儿茶素和原花青素B3。

To develop a new utilization for Japanese cedar (Sugi : Cryptomeria japonica D.Don), we inverstigated the bioactivities of sugi extractives, superoxide dismutase (SOD) mimic and angiotensin I-converting enzyme (ACE) inhibitory activities. The ethanol extract from heartwood and outer bark showed the highest SOD mimic sctivity and ACE inhibitory activity, respectively, among 24 extracts prepared from roots, leaves, heartwood, sapwood, inner bark, and outer bark by successive extraction with n-hexane, chloroform, ethanol, and hot water. Isolation of active compounds from each extract was attempted by HPLC equipped with a reversed phase column, and identification of the bioactive compound was achieved by GC-MS and ^1H-NMR.As the results, we isolated and identified sequrin-C from the heartwood as a SOD mimic active compound, and catechin and procyanidin B3 as ACE inhibitors from the outer bark.