Effects of organotin compounds and lectins on DNA

1)培养细胞：白血病和乳腺肿瘤细胞系在含100%FBS的RPMI-1640培养液中维持。2)有机锡和凝集素对培养细胞增殖的影响：有机锡具有特异性毒性作用，对淋巴系细胞的影响大于对髓系细胞的影响。未成熟的细胞可能比成熟的细胞对有机锡更敏感。3)有机锡和凝集素对Friend细胞分化的影响DMSO和二丁基锡的混合物可明显抑制DMSO对Friend细胞的分化，且抑制率与DMSO和二丁基锡的混合时间有关。4)我们从兔血清中分离到一种新的磷脂/神经节苷脂结合蛋白。凝集素能凝集兔红细胞。这些凝集素具有相同多肽链上的胶原样序列，对细菌胶原酶消化具有抵抗力。5)培养细胞DNA提取和尿8-羟色胺测定：从培养细胞中提取总DNA，用地高辛标记法检测，灵敏度高。8-OHdG经商品色谱柱离子交换层析后，用高效液相色谱-电化学法进行检测。但尿液中添加的8-OHdG的回收率约为20-30%，尿液的前处理方法有待进一步研究。

Effects of organotins arid lectins on DNA were investigated in cell lines.1) Cultured cells : leukemia and mammary tumor cell lines were maintained in RPMI-1640 medium containing 100%FBS.2) Effects of organotins and lectins on proliferation of cultured cells : organotins exhibited a specific toxic effect and the effect on lymphoid cells was stronger than on myeloid cells. Immature cells may be more sensitive to organotins than mature cells. MCF-7 (mammary tumor cell line) was proliferated at levels of 10^<-10>M order of tributyltin which cell viability was not yet impaired.3) Effects of organotins and lectins on differentiation of Friend cell the differentiation of Friend cell by DMSO was markedly inhibited by treatment with mixture of DMSO and dibutyltin and the inhibitory rate was dependent on the mixing time of DMSO and dibutyltin.4) We have isolated a novel phospholipid / ganglioside-binding protein from rabbit sera. The lectin agglutinated rabbit red cells. These lectins have collagen-like sequences in the same polypeptide chain, and are resistant to bacterial collagenase digestion.5) Ditection of extracted DNA from cultured cells and determination of 8-OHdG in urine : total DNA was extracted from cultured cells and detected by DIG method in high sensitivity. After using an ion exchange chromatography of a commercial column, the 8-OHdG was detected by HPLC with electric-chemical detector. But the recovery of added 8-OHdG in urine was about 20 - 30%, so the pretreatment method of urine must be further investigated.