POSTMORTEM CHANGES OF VOLATILE COMPOUNDS DETECTED IN DECOMPOSED TISSUES

1.腐烂组织中煤油成分的测定毛细管气相色谱/质谱仪(GC/MS)与正戊烷溶剂萃取法相结合，建立了一种灵敏、快速的分析腐烂组织中煤油成分的方法。切开后，置于室温下，时间为0~24小时。直到死后24小时，组织中才能清晰地检测到碳数为9~20的脂肪烃以及异丙苯、异丙苯、均三甲苯和1，2，3-三甲苯等三甲苯。2.腐烂组织中煤油组分的死后变化1)组织中三甲苯的浓度变化幅度为40%~110%。死后6h，肾、肝、脑等脂肪丰富的器官中的三甲苯含量逐渐升高。2)在实验范围内，肺和血液中的三甲苯浓度比在0.4~1.4之间。3)死后24小时内，大腿肌肉中的三甲苯含量基本稳定，因此可以根据大腿肌肉中的三甲基苯的值来推断血液中因分解而无法校正的煤油成分的浓度。

1.Determination of kerosene components in putrefied tissuesA sensitive and rapid method to analyze derosene components in decomposed tissues was developed by making use of capillary gas chromatography/mass spectrometry (GC/MS) combined with the solvent extraction method using n-pentane.Rats were exposed to 1,000 ppm kerosene vapor for 15 min. After pithing, the rats were left at room temperature during from 0 to 24 hrs. The aliphatic hydrocarbons with carbon number from 9 to 20 and trimethylbenzenes such as cumene, pseudocumene, mesitylene and 1,2,3-trimethylbenzene were clearly detected in tissues until 24 hrs after death.2.Postmortem changes of levels of kerosene components in decomposed tissues1) The concentrations of trimethylbenzenes in tissues were changed in a range of 40 to 110% against the initial levels. The level of trimethylbenzenes in fat riched organ such as kidney, liver and brain were gradually increased from 6 hrs after death due to postmortem distribution.2) The concentration ratios of trimethylbenzenes in lung to blood were from 0.4 to 1.4 within the examined.3) The levels of trimethylbenzens in thigh muscle were nearly stable within 24 hrs after death, so that the concentration of kerosene components in blood which could not have been corrected due to decomposition would be inferred from the values of trimethylbenzines in thigh muscle.