Spectral 5-Laser Cell Sorter

作为波恩大学医学系流式细胞仪核心设备的现有设备的扩展，将采购一个安装在生物安全柜中的高参数、光谱、高速细胞分选系统。在过去的几年里，流式细胞仪核心设备对细胞分选的要求稳步增加。随着2022年初频谱分析流式细胞仪的问世，对超过20个参数的细胞分选的需求急剧增加。目前，核心设施只有一个细胞分选系统，该系统安装在生物安全柜中，配备了5个激励激光器，最多可分选20个参数。购买该设备主要是为了分选生物安全级别2的细胞，位于S2实验室，主要用于生物安全级别2的生物体分选。对于已经存在的和未来计划的不同学科的研究项目，需要额外的设备，该设备能够分选20多个参数，配备紫外线激发激光器，能够处理光谱数据，并且能够收集多达6个目标种群。此外，有必要将该设备安置在生物安全工作台中，以便也可以对未经测试的人体样本进行分类。

As an extension to the existing equipment of the Flow Cytometry Core Facility of the Medical Department of the University Bonn, a high-parameter, spectral, high-speed cell sorting system housed in a biosafety cabinet is to be procured. Cell sorting requirements have increased steadily in the Flow Cytometry Core Facility over the past several years. With the acquisition of a Spectral Analytical Flow Cytometer in early 2022, the demand for cell sorting beyond 20 parameters is increasing tremendously. Currently, the Core Facility has only one cell sorting system, which is housed in a biosafety cabinet and equipped with 5 excitation lasers, that can sort a maximum of 20 parameters. This device was purchased primarily for sorting cells of biosafety level 2, is located in an S2 laboratory, and is largely utilized solely for sorting organisms of biosafety level 2. For already existing and future planned research projects from different disciplines an additional device is needed, which is able to sort more than 20 parameters, equipped with a UV excitation laser, able to process spectral data and which is able to collect up to 6 target populations. Furthermore, it is necessary that the device is housed in a biosafety workbench so that untested, human samples can also be sorted.