Clinicopathologic and molecular biologic study on histogenesis and development of colorectal carcinoma

结直肠癌的组织发生和发展：我们检测了息肉样（P）和浅表（S）型结直肠癌的Ki-67标记指数（LI）、细胞凋亡、MUC-1和pS2蛋白。1)Ki-67 LI在S型病变中明显高于P型癌。P型癌细胞凋亡率明显高于S型癌。结果表明S型和P型大肠癌的增殖行为不同。2)结直肠癌显示MUC-1表达的高发生率，然而，结直肠腺瘤显示几乎为零的MUC-1表达的发生率。对于浸润性癌，MUC-1表达与浸润/转移潜能和预后相关。提示MUC-1的表达与结直肠癌的发生密切相关，是预测结直肠癌侵袭转移能力和预后的一个有用指标。3)结直肠癌和增生性病变的发生率明显高于非癌性病变。 ...更多信息 S2蛋白表达高于腺瘤。pS2蛋白在息肉样、较大、位于右半结肠及腺瘤样病变中的表达率明显高于正常对照组。pS2蛋白被怀疑与结直肠癌的发生有关，考虑到与其他各种因素的关系，需要进一步的研究。内镜切除术后残留结直肠癌的潜在恶性：我们检查了高频电灼圈套切除术在裸鼠移植的结直肠癌中的刺激效果。Ki-67 LI的评估显示，高频电灼圈套切除术的刺激使肿瘤获得了比切除前更高的增殖活性。高频电灼圈套器切除刺激的几种肿瘤中的TGF-α和EGF受体表达显示出比切除前更强的强度。而VEGF、bFGF和IV型胶原酶的表达强度在手术前后无明显变化。提示内镜切除术后残留的部分肿瘤在高频电灼圈套器切除的刺激下，可获得较高的增殖活性。少

Histogenesis and development of colorectal carcinoma : We examined Ki-67 labeling index (LI), apoptosis, MUC-1 and pS2 protein in both polypoid (P) and superficial (S) type of colorectal carcinoma. 1) Ki-67 LI in S type lesions was significantly higher than that in P type carcinomas. Apoptosis in P type lesions was significantly higher than that in S type carcinomas. These results show that S and P type of colorectal carcinoma are different in poliferative behavior. 2) Colorectal carcinomas revealed a high incidence of MUC-1 expression, however, colorectal adenomas showed almost zero incidence of MUC-1 expression. Regarding invasive carcinoma, MUC-1 expression is related to invasive/metastatic potential and prognosis. These results indicated that MUC-1 expression closely contributed to colorectal carcinogenesis and a useful indicator in predicting the invasive/metastatic potential and prognosis. 3) Colorectal carcinoma and hyperplastic lesions showed significantly higher incidence of p … More S2 protein expression than adenoma. pS2 protein showed a significantly higher incidence in lesions with polypoid appearance, lager size, location of right side colon and adenomatous lesion. pS2 protein suspected to be related to colorectal carcinogenesis and further studies are needed considering the relation to other various factors.Mlignant potential of residual colorectal carcinoma after endoscopic resection :We examined the effect of stimulation by high frequent electrocautery snare resection in colorectal carcinoma implanted in nude mouse. Evaluation of Ki-67 LI revealed that stimulation by high frequent electrocautery snare resection had the tumor acquired a higher proliferative activity than before resection. TGF-alpha and EGF receptor expression in several tumors stimulated by high frequent electrocautery snare resection showed a stronger intensity than those before resection. However, the intensity of VEGF,bFGF and type IV collagenase expression showed no significant change before and after resection. These results indicated that some of residual tumor after endoscopic resection may acquire a higher proliferative activity by stimulation by high frequent electrocautery snare reseciton. Less