Study on cell proliferation in the gallbladder and bile duct epithelium with pancreaticobiliary maljinction.

我们成功地制作了一种新的胰胆畸形无胆管扩张模型，并证实了该模型胆囊细胞周期和胆管上皮细胞周期的增加。对9只动物进行胆胰侧侧导管吻合，建立猫模型。6只只暴露胆总管-胰管的猫作为对照。1年后，切除这些猫的胆总管，用抗增殖细胞核抗原（PCNA）抗体（PC10, Dako）染色。标记指数（LI）由三个显微镜视野中阳性细胞核的百分比确定。组织学上，与对照组相比，模型中的管周腺发育良好。在模型中，胆管与腺体连接管处PCNA阳性细胞数量明显增加。平均值(()+-。[)标准偏差]PCNA LI为27.2 (]SY.+-。[) 10.9%的模型和8.3 (]SY.+-。对照组为4.2% （p<0.01）。组织学上上皮内陷，囊泡增多，囊周腺发育良好。PCNA阳性细胞在总胆管中显著增加，尤以胆管小囊和腺体中明显增加。这些数据清楚地表明，细胞周期增加，不仅在胆囊，而且在总胆管，建议切除胆管在这种疾病。

We have succeeded in making a new model for the pancreaticobiliary maljunction without bile duct dilatation, and demonstrated increased cell cycle of the gallbladder and the bile duct epithelium of this model.A cat model was produced by performing choledocho-pancreatic side-to-side ductal anastomosis in nine animals. Six cats, in which the choledocho-pancreatic ducts were exposed only, served as controls. After 1 year, the common bile duct of these cats were removed and stained with anti-proliferating cell nuclear antigen (PCNA) antibody (PC10, Dako). The labeling index (LI) was determined from the percentage of positive nuclei in three microscopic fields.Histologically periductal glands are well developed in models compared to controls. In the models, the number of PCNA positive cells was significantly increased at the connecting duct between the bile duct and glands. The mean ((]SY.+-。[) standard deviation) PCNA LI was 27.2 (]SY.+-。[) 10.9% in the models and 8.3 (]SY.+-。[) 4.2% in the controls (p<0.01). Histologically invaginations of the epithelium, sacculi of Beale increased in number and periductal glands were well developed. PCNA positive cells significantly increased in the common bile duct, especially in sacculi of Beale and glands.These data clearly indicate that the cell cycle is increased not only in the gallbladder but also in the common bile duct and that excision of the bile duct is recommended in this disease.