Neurotrophic Effects of Cytokine-activated Astrocytes.-Basic research for Elucidation of Interaction between Neuron and Glia, and Restoration of Damaged Neuronal Function-

我们已经证明，星形胶质细胞产生和分泌神经营养因子如神经生长因子（NGF）可被细胞因子如成纤维细胞生长因子（FGF）、白细胞介素-1 β（IL-1 β）（IL-1 β）、肿瘤坏死因子-α（TNF-α）、表皮生长因子（EGF）、转化生长因子-β 1（TGF-β 1），这是可能存在于脑损伤后。我们已经证明了新的知识如下所述，从1996年至1998年。结论1. IL-1 β、TNF-α和EGF诱导培养的大鼠海马星形胶质细胞高分子量bFGF亚型选择性增加. IL-1 β和TNF-α协同增强星形胶质细胞对脑桥胆碱能神经元的一种新的营养活性. TGF-β 1增强培养的大鼠海马星形胶质细胞NCAM的表达.培养的大鼠海马星形胶质细胞表达肝细胞生长因子受体c-Met。细胞因子增强培养大鼠海马星形胶质细胞c-Met蛋白表达

We have demonstrated that production and secretion of neurotrophic factors such as nerve growth factor (NGF) from astrocytes was enhanced by cytokines such as fibroblast growth factor(FGF), interleukin-1beta(IL-1beta), tumor necrosis factor-alpha(TNF-alpha), epidermal growth factor(EGF), transforming growth factor-beta1(TGF-beta1), which are likely to be present in the brain following injury.We have demonstrated new knowledge mentioned below, from 1996- to 1998.1. IL-1beta, TNF-alpha and EGF induced selective increase of high-molecular-weight bFGF isoforms in cultured rat hippocampal astrocytes.2. IL-1beta and TNF-alpha co-operatively enhanced a novel trophic activity of astrocytes for pontine cholinergic neurons.3. TGF-beta1 enhanced expression of NCAM in cultured rat hippocampal astrocytes.4. Hepatocyte growth factor(HGF) receptor, c-Met was expressed in cultured rat hippocampal astrocytes. Cytokines enhanced expression of c-Met protein in cultured rat hippocampal astrocytes.