Relationships between diabetes mellitus and oral bacteria in periodontitis.

血糖控制对链脲佐菌素诱导的糖尿病大鼠实验性牙周炎的影响胰岛素治疗对常规大鼠和无菌大鼠的影响探讨血糖控制对链脲佐菌素诱导的实验性牙周炎大鼠牙周状况的影响。常规大鼠和无菌大鼠分为正常大鼠和胰岛素治疗大鼠。在右侧下颌第一磨牙周围放置一个弹性环。在第14天和第28天对牙周状况进行评估。记录血糖水平、炎症变化程度及牙槽骨面积的定量测量。弹性环在牙周间隙内植入会导致附着体丧失和牙槽骨吸收。非糖尿病组和糖尿病-胰岛素对照组实验侧牙槽骨面积在第14和28天均显著大于糖尿病组。牙周病细菌对HL-60和人牙龈成纤维细胞超氧化物相关酶的诱导作用将HL-60和成纤维细胞与牙周病细菌培养。以超氧化物歧化酶和过氧化氢酶的存在程度评价HL-60，以一氧化氮合酶的存在程度评价成纤维细胞。采用fito间接免疫抗体法对各酶进行染色。超氧化物歧化酶和过氧化氢酶在所有细胞中广泛分布。一氧化氮合酶在小范围内扩散。

Effects of Blood Glucose Control on Experimental Periodontitis in Streptozotocin-induced Diabetic Rats Effects of Insulin Therapy in Conventional and Germ Free RatsIt was evaluated the effect of the glycemic control on the periodontal condition of streptozotocin-induced diabetic rats with experimental periodontitis. Conventional and germ-free rats were divided into normal rats and insulin-treated rats. An elastic ring was placed around the right mundibular 1St molar. The evaluation of the periodontal condition was made on 14 and 28 days. Blood glucose level, the extent of the inflammatory change, and the quantitative measurement of the alveolar bone area were recorded. The insertion of elastic ring in the periodontal crevice induced attachment loss and alveolar bone resorption. The alveolar bone area of the experimental side in the non-diabetes mellitus group and diabetes mellitus-insulin control group were significantly larger than in the diabetes mellitus group on 14 and 28 days.Induction of Superoxide related enzymes in HL-60 and human gingival fibroblasts stimulated by periodonto-pathic bacteriaThe HL-6O and fibroblasts were incubated with periodonto-pathic bacteria. The HL-60 was evaluated the existence of Superoxide Dismutase and catalase , fibroblasts was estimated by the existence of nitric oxide synthase in each cell. Each enzyme was stained by FITO-laveled indirect-immunological antibody method. The superoxide dismutase and catalase were widely spread in the all cells. The nitric oxide synthase was spread in small area.