The assaying methods for the free radicals and active oxygen species arising from food substances in trace amounts and their scavenging

令人遗憾的是，首席调查员的严重疾病在很大程度上阻碍了调查的进展和最终报告。1)本研究致力于开发一种新的检测食品中产生的痕量活性氧物种和自由基的方法。为此，进一步改进了一种极其灵敏的单光电子计数方法。2)在热变性的蛋白质食品中，如肉类和鱼类，检测到最早产生一系列活性氧物种的活性氧的超氧化物。3)烟熏食品也产生超氧化物，但有微量。4)即使牛排的烧焦部分也产生超氧化物。5)以葡萄糖为代表的热变性碳水化合物，在适当加热时产生超氧化物。在实验体系中，过氧化氢的产生和积累伴随着超氧化物的产生。6)由于测量系统的改进，在糖和糖-氨基酸混合物的溶液中检测到了超氧化物和过氧化氢的产生。7)白蛋白溶液中的香烟烟雾显示了超氧化物的产生。8)多酚类物质普遍存在于水果和蔬菜中，有效地阻止了上述超氧化物和过氧化氢的产生。

It is quite regretful that a serious sickness of the head investigator has largely hampered the progress and the final report of this investigation.1)This research pursues away to develop a new detecting method of trace amounts of active oxygen species and free radicals generated in food substances. For this purpose an extremely sensitive single photoelectron counting method was further refined.2)Superoxide, the firstly produced active oxygen of a series of active oxygen species, was detected in the heat-denatured proteinous food substances such as meats and fishes.3)Smoked foods also produced superoxide, though in trace amounts.4)Even the charred portions of beefsteaks generated superoxide.5)Heat-denatured carbohydrates, represented by glucose, produced superoxide when appropriately heated. The generation and accumulation of hydrogen peroxide followed the production of superoxide in the experimental system.6)As the result of refinement of measuring system, the generations of superoxide and hydrogen peroxide have been detected in the solutions of sugars alone, and sugar-amino acid mixtures.7)Cigarette smoke absorbed in albumin solution showed the generations of superoxide.8)Polyphenols, generally residing in fruits and vegetables, effectively blocked the generations of superoxide and hydrogen peroxide above mentioned.