Study of Helicobacter pylori infection using G-CSF transgenic mice.

1.各种细菌gnotobiot小鼠的定殖能力：使用gnotobiot小鼠检查胃、肠和口腔中各种肠细菌和幽门螺杆菌的定殖，因为据报道幽门螺杆菌的定殖在常规小鼠中被正常细菌植物群抑制。将11株细菌接种于无菌小鼠。这些细菌可以在胃、肠和口腔中定植。但不同菌株在不同地点的细菌数量存在差异。如胃内可分离出大量幽门螺杆菌，而口腔内仅分离出少量.转基因小鼠中幽门螺杆菌的感染：转基因小鼠接种幽门螺杆菌悉尼菌株1（SS 1）。该菌株可以在这些小鼠的胃中定殖。感染后10周，SS 1数量减少。感染后2周，胃粘膜出现炎症反应，比对照组早.预防措施3.幽门螺杆菌脂多糖可诱导内皮细胞合成细胞因子。

1. Colonization ability of various bacteria gnotobiot mice :Colonization of various enterobacteria and Helicobacter pylori in the stomach, intestine and oral cavity was examined using gnotobiot mice, because colonization of Helicobacter pylori has been reported to be inhibited by normal bacterial flora in conventional mice. Eleven bacterial strains were inoculated to germ free mice. These bacteria could colonize in stomach, intestine and oral cavity. However, number of bacteria in each site was various among bacterial strain. For example, large number of Helicobacter pylori could be isolated from stomach while only small number of it could isolated from oral cavity.2. Infection of Helicobacter pylori in transgenic mouse: Transgenic mice were inoculated with Helicobacter pylori Sydney strain 1 (SS1). The strain could colonize in the stomach of these mice. The number of SS1 was decreased at 10 weeks after infection. The inflammation in the stomach was appeared at 2 week after infection/ It is earlier than that in control mice.2. Infection3. Lypopolysaccharide from Helicobacter pylori could induce cytokine synthesis from endothelial cells.