The investigation on the relation between the changes of tumor glucose metabolism and the apoptosis after irradiation-evaluated by measuring the lactic acid using ^1H-MRS-

恶性肿瘤的糖代谢普遍增高，无氧糖代谢优于有氧代谢。乳酸是无氧代谢的主要代谢物。通过测量乳酸，他们应该能够评估肿瘤的活性。为了阐明辐照后肿瘤糖代谢变化与细胞凋亡的关系，我们开展了这项研究。用^1H-MRS测量小鼠移植的放射诱导纤维肉瘤（RIF-1）的乳酸浓度。淋巴瘤瘤内脂质含量丰富，移植到裸鼠的人淋巴瘤的乳酸浓度无法用^1H-MRS测量。计算葡萄糖注射前后RIF-1肿瘤乳酸浓度之比，作为肿瘤糖代谢的标准。照射（20Gy） 6小时后，乳酸比率无明显变化。照射12小时后，乳酸比率下降。H-E染色组织学评价，照射24小时后无明显变化。由此看来，可以用^1H-MRS法预测乳酸盐的辐射效应。在RIF-1肿瘤中，TUNEL法未证实凋亡的出现。

Glucose metabolism of malignant tumor is generally increased, and anaerobic glucose metabolism is superior to the aerobic metabolism. Lactate is a main metabolite by the anaerobic metabolism. By measuring thelactate, they should be able to evaluate the activity of the tumor.We carried out this research in order to clarify the relationship between the changes of tumor glucose metabolism and the apoptosis after irradiation.The lactate concentration of the radiation induced fibrosarcoma (RIF-1) transplanted to the mouse was measurable by using ^1H-MRS.The lipid content of the intratumorale was abounding for the lymphoma, the lactate concentration of the human lymphoma transplanted to the nude mouse was not able to be measured by ^1H-MRS.The ratio of the lactale concentration of the RIF-1 tumor before and after glucose injection was calculated and was used as a standard of the glucose metabolism of the tumor. There was no change of the lactate ratio by after 6 hours of the irradiation (20Gy). The lactate ratio is lowering after 12 hours of the irradiation. In the histological evaluation by a H-E stain, there was no change by after 24 hours of the irradiation.From this result, it seemed to be possible to predict the radiation effect from lactate evaluation by ^1H-MRS.In the RIF-1 tumor, the appearance of the apoptosis was not confirmed by TUNEL method.