Role of Chemokines in Acute Rejection after Rat Lung Transplantation. Interaction with Nitric Oxide and Endothelin.

大鼠肺移植分为同系和同种异体两种。在同种异体移植物中，没有发现急性排斥反应，也没有发现NO产生的显著增加。在同种异体移植物中，术后第3天（POD）观察到I-II级急性排斥反应，术后第5天观察到III级。BAL细胞产生的NO在POD 3（11.8±2.0 ppb）和POD 5（115.3±66.9 ppb）均增加。NO水平与AR的严重程度呈显著正相关（p<0.005）。免疫组化结果显示，同种异体移植物的BAL细胞表达iNOS mRNA，巨噬细胞、淋巴细胞和中性粒细胞表达iNOS。因此，它可以作为肺移植AR的敏感指标。

Rat lung transplantation was performed in isografts and allografts. In isografts, no acute rejection and no significant increase in NO production was identified. In allografts, grade I-II of acute rejection was seen on postoperative day (POD) 3 and grade III on POD 5. NO produced by BAL cells increased on both POD 3 (11.8±2.0 parts per billion [ppb] and POD 5 (115.3±66.9 ppb). There was a highly significant correlation between the level of NO and the severity of AR (p<0.005). BAL cells from allografts expressed iNOS mRNA.Among them, macrophages, lymphocytes and neutrophils were immunostained for iNOS.In conclusion, NO production by BAL cells was detected in the early stages of rejection. Therefore it may serve as a sensitive indicator of AR in lung transplantation.