Fundamental research of the effect of a variety of chemotherapeutics on leukocyte functions

巨噬细胞和中性粒细胞在自然宿主防御系统中起着重要作用。当这些吞噬细胞识别外来颗粒，如细菌和真菌时，它们会逃离血管系统，向感染部位迁移，然后吞噬并通过超氧阴离子产生的杀菌活性氧物种在细胞内杀死入侵的微生物。为了确定抗菌药是否影响这些功能，我们检测了氧氟沙星(OFLX)、洛美沙星(LFLX)、妥舒沙星(FLRX)、氟罗沙星(FLRX)、司帕沙星(SPFX)和左氧氟沙星(LVFX)这6种新的喹诺酮类药物对大鼠外周巨噬细胞和中性粒细胞功能的影响。新的喹诺酮类药物在体外处理后能显著增强巨噬细胞产生超氧阴离子。TFLX对超氧阴离子产生的增强作用延长，但对OFLX、LFLX、FLRX、SPFX和LVFX的增强作用是短暂的。相反，Tflx显著增强了巨噬细胞…的粘附性更多的年代。其他新喹诺酮类药物对巨噬细胞的黏附无明显影响。此外，所有新的喹诺酮类药物都显著抑制了巨噬细胞对大肠杆菌的趋化和吞噬作用。此外，OFLX、LFLX、TFLX和LVFX均能显著增强巨噬细胞过氧化氢的产生，而其他药物对其无明显影响。相比之下，OFLX、LFLX、FLRX和LVFX均能增加中性粒细胞对大肠杆菌的吞噬作用。然而，TFLX和SPFX未能显著影响中性粒细胞的吞噬功能。此外，LFlx和SPFX显著增强中性粒细胞的粘附性。TFLX对中性粒细胞产生超氧阴离子有增强作用，而其他五种新的喹诺酮类药物有效地显著减少了中性粒细胞超氧阴离子的产生。此外，TFLX能显著增强中性粒细胞过氧化氢的产生，而司帕沙星则显著抑制这种产生。因此，新的喹诺酮类药物可能会对巨噬细胞和中性粒细胞的功能产生不同的影响。较少

Macrophages and neutrophils play an important role in natural host defense system. When these phagocytes recognize foreign particles, such as bacterium and fungi, they escape the vascular system, migrate towards the site of infection, followed by phagocytosis and intracellular killing of the invading microorganisms by bactericidal reactive oxygen species derived from the superoxide anion. To determine whether antimicrobials affect these functions, we examined the effect of 6 new quinolones, ofloxacin (OFLX), lomefloxacin (LFLX), tosufloxacin (FLRX), fleroxacin (FLRX), sparfloxacin (SPFX) and levofloxacin (LVFX) on the function of rat peripheral macrophages and neutrophils. In vitro treatment of the new quinolones was effective in markedly potentiating the production of superoxide anion in macrophages. The potentiation of the production of superoxide anion is prolonged with TFLX but transient for OFLX, LFLX, FLRX, SPFX and LVFX. In contrast, TFLX markedly potentiated adhesion in macroph … More ages. No significant alteration was detected in the adhesion in macrophages treated with the other new quinolones. In addition, all of the new quinolones significantly inhibited chemotaxis and phagocytosis of E. coli in macrophages. Moreover, OFLX, LFLX, TFLX, and LVFX were effective in significantly potentiating the production of hydrogen peroxide in macrophages, while the other agents did not affect that. By contrast, OFLX, LFLX, FLRX and LVFX caused an increase of phagocytosis of E. coli in neutrophils. However, TFLX and SPFX failed to significantly affect phagocytosis in neutrophils. Furthermore, LFLX and SPFX significantly potentiated adhesion of neutrophils. A potentiation by TFLX was seen with the production of superoxide anion in neutorphils, while the other five new quinolones were effective in markedly reducing that production in neutrophils. Moreover, TFLX was effective in significantly potentiating the production of hydrogen peroxide in neutrophils, whereas sparfloxacin markedly inhibited such production. Therefore, the new quinolones may differentially affect the function of macrophages and neutrophils. Less