Study on the three dimensional calcification around the surface of biomaterial granules using bovine osteoblastic primary culture

本研究的目的是利用成骨细胞原代培养的方法研究几种生物材料表面的三维骨形成。然而，由于包括胎牛骨细胞在内的成骨细胞原代培养的几个限制，我刚刚发现，从猪胚胎下颌骨衍生的成骨细胞原代培养物在某些情况下显示基质钙化。当商业上可获得的从人骨衍生的成骨细胞原代培养物与羟基磷灰石颗粒一起培养时，它显示了在二维培养物中看不到的有趣结构。这意味着成骨细胞原代培养可能是研究生物材料颗粒共培养时体内三维骨形成机制的有力工具。但其存在的最大问题是细胞增殖分化缓慢，存活率低，因此，本研究开发了一种新的猪胚下颌骨成骨细胞原代培养方法。该培养体系在某些情况下可在周围基质中快速钙化。基质快速钙化的最佳条件仍需阐明。

The aim of this study was to investigate on three dimensional bone formations on the surface of several biomaterials using osteoblastic primary culture. However, because of several restrictions regarding osteoblastic primary culture, including fetal bovine bone cells, I've just found that osteoblastic primary culture derived from embryonic porcine mandibular bone shows matrix calcifications under some circumstances so far.When commercially available osteoblastic primary culture derived from human bone was cultured with hydroxyl apatite granules, it showed interesting structures that was not seen in the two dimensional cultures. This means osteoblastic primary cultures may be powerful tools to study the mechanisms of three dimensional bone formations in vivo when co-cultured with biomaterial granules. However, the biggest problem was that proliferation and differentiation of those cells were very slow and the viability of those was too low.Now, a new osteoblastic primary culture derived from embryonic porcine mandibular bone was developed. This culture system showed rapid calcification among surrounding matrix under some circumstances. Elucidation of the optimal conditions for rapid calcification of matrix still needs to be done.