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Investigation on mechano-receptor in the cell using rat periodontal ligament

利用大鼠牙周膜研究细胞内的机械感受器

基本信息

批准号：
16390551
负责人：
UOSHIMA Katsumi
金额：
$ 6.44万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390551/
关键词：
occulusal trauma periodontal ligament imunohistochemistry osteopontin

项目摘要

The purpose of this research was to establish the animal experiment model for the investigation of periodontal ligament response to the dynamic stress, and to find mechano-receptor in the periodontal ligament cells that convert a physical stress of occlusal load to a biochemical signal.In 2004, we tried to establish an animal model. Through bonding 1 mm in diameter wire to the rat upper molar, histological changes in periodontal ligament could be investigated that was simulating occlusal trauma. As a result, the time that the periodontal ligament converted from the destruction process to the regenerative process was able to be retrieved in detail.In addition, we selected several kinds of proteins related to the bone, and immunohistochemistry was done using the antibody to these. As a result, the production of Osteopontin was up regulated in osteoclasts. Then we tried to find RANKL production because it is thought to be working as the upstream cytokine, but we could not. This means there might be a specific pathway through which Osteopontin production is controlled.In 2005 and 2006, we investigated histological changes of periodontal ligament under no loading condition. Because loading machine was not available, we selected the temperature stimulus instead of mechanical stimulation. As a result, the common feature of a temperature and mechanical stimulation cannot be found. Further study is required to compare these more precisely.In 2007, we tried to establish animal model that enables us to investigate thermal influences to the periodontal ligament, but we could not achieve because we could not fabricate a handy device for this purpose. Further study is necessary.

本研究的目的是建立动物实验模型来研究牙周膜对动态应力的反应，并寻找牙周膜细胞中将咬合负荷的物理应力转化为生化信号的机械感受器。2004年，我们尝试建立动物模型。通过将直径1毫米的金属丝粘合到大鼠上磨牙上，可以模拟牙合创伤来研究牙周膜的组织学变化。结果，能够详细检索牙周膜从破坏过程转变为再生过程的时间。此外，我们选择了几种与骨相关的蛋白质，并使用这些抗体进行了免疫组织化学分析。结果，破骨细胞中骨桥蛋白的产生上调。然后我们试图找到 RANKL 的产生，因为它被认为是上游细胞因子，但我们找不到。这意味着可能存在控制骨桥蛋白产生的特定途径。2005年和2006年，我们研究了无负荷条件下牙周膜的组织学变化。由于没有装载机，我们选择了温度刺激而不是机械刺激。结果，无法找到温度和机械刺激的共同特征。需要进一步的研究来更精确地比较这些。2007年，我们试图建立动物模型，使我们能够研究热对牙周膜的影响，但我们未能实现，因为我们无法为此目的制造方便的设备。有必要进一步研究。