Investigation on mechano-receptor in the cell using rat periodontal ligament

利用大鼠牙周膜研究细胞内的机械感受器

基本信息

批准号：
16390551
负责人：
UOSHIMA Katsumi
金额：
$ 6.44万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390551/
关键词：
occulusal trauma periodontal ligament imunohistochemistry osteopontin

项目摘要

The purpose of this research was to establish the animal experiment model for the investigation of periodontal ligament response to the dynamic stress, and to find mechano-receptor in the periodontal ligament cells that convert a physical stress of occlusal load to a biochemical signal.In 2004, we tried to establish an animal model. Through bonding 1 mm in diameter wire to the rat upper molar, histological changes in periodontal ligament could be investigated that was simulating occlusal trauma. As a result, the time that the periodontal ligament converted from the destruction process to the regenerative process was able to be retrieved in detail.In addition, we selected several kinds of proteins related to the bone, and immunohistochemistry was done using the antibody to these. As a result, the production of Osteopontin was up regulated in osteoclasts. Then we tried to find RANKL production because it is thought to be working as the upstream cytokine, but we could not. This means there might be a specific pathway through which Osteopontin production is controlled.In 2005 and 2006, we investigated histological changes of periodontal ligament under no loading condition. Because loading machine was not available, we selected the temperature stimulus instead of mechanical stimulation. As a result, the common feature of a temperature and mechanical stimulation cannot be found. Further study is required to compare these more precisely.In 2007, we tried to establish animal model that enables us to investigate thermal influences to the periodontal ligament, but we could not achieve because we could not fabricate a handy device for this purpose. Further study is necessary.

这项研究的目的是建立动物实验模型，以研究牙周韧带对动态应激的反应，并在牙周韧带细胞中找到机械受体，以将咬合负荷的物理应力转化为生化信号。在2004年，我们试图建立动物模型。通过将直径1毫米粘结到大鼠上摩尔的粘结，可以研究牙周韧带的组织学变化，以模拟咬合创伤。结果，可以详细检索从破坏过程转化为再生过程的牙周韧带的时间。此外，我们选择了与骨骼相关的几种蛋白质，并使用对这些抗体进行了免疫组织化学。结果，骨桥蛋白的产生在破骨细胞中受到调节。然后，我们试图找到RANKL生产，因为人们认为它是上游细胞因子的作用，但我们不能。这意味着可能会有一种特定的途径控制骨桥蛋白的产生。在2005年和2006年，我们研究了在无负荷条件下牙周韧带的组织学变化。由于没有加载机，我们选择了温度刺激而不是机械刺激。结果，找不到温度和机械刺激的共同特征。需要进一步的研究来更精确地比较它们。在2007年，我们试图建立动物模型，使我们能够研究对牙周韧带的热影响，但是我们无法实现，因为我们无法为此目的制造便捷的装置。进一步的研究是必要的。