非金属歯科材料アレルギーを対象としたパッチテスト用試薬開発とその基礎に関する研究

本研究的目的是建立用于贴片试验的牙科材料非金属试剂，并研究其对这些抗原的生物反应。首先，我们试图分析一些特定牙科材料的成分。然而，我们在6个月后未能做到这一点。由于时间紧迫，我们决定在过敏患者中使用“现状”牙科材料（如水泥和树脂）进行贴片试验。65例患者使用“原样”材料进行检测，24例患者使用单一试剂进行检测。另外10名对照组非过敏志愿者也接受了“原样”材料的测试。结果，在过敏患者中有大量的斑贴试验阳性，甚至一个健康的志愿者也显示出严重的阳性。我们必须从现在开始研究这些非金属牙科材料过敏。为了研究过敏患者与健康个体之间的遗传差异，我们收集了5名志愿者的外周血并比较了蛋白质组。结果，我们发现了大约10种可能在患者过敏反应中起一定作用的蛋白质。

The purpose of this study was to establish non-metallic reagents of dental materials for patch tests and to investigate the biological responses against those antigens.1.First, we tried to analyze the constituents of some specific dental materials. However, we failed to do so after 6 month or so. Because of the lack time, we decided to perform patch tests among allergic patients using "as is" dental materials such as cements and resins. 65 patients were tested with "as is" materials and 24 patients were tested with single reagents. 10 control non-allergic volunteers were tested with "as is" materials as well. As a result, there were significant numbers of patch test positives among the allergic patients and even a healthy volunteer showed severe positive a resin. We have to study these non-metallic dental material allergies from now on.2.To study genetic differences between allergic patients and healthy individuals, we collected peripheral bloods out of 5 volunteers and proteomes were compared. As a result, we found approximately 10 putative proteins that might be playing some roles in the allergic reactions in the patients.