非金属歯科材料アレルギーを対象としたパッチテスト用試薬開発とその基礎に関する研究

非金属牙科材料过敏斑贴试验试剂的研制及基础研究

• 批准号: 13557164
• 负责人: UOSHIMA Katsumi
• 金额: $ 3.84万
• 依托单位: NIIGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13557164/
• 关键词: non-metallic; patch test; proteomics; allergy; 抗原性

The purpose of this study was to establish non-metallic reagents of dental materials for patch tests and to investigate the biological responses against those antigens.1.First, we tried to analyze the constituents of some specific dental materials. However, we failed to do so after 6 month or so. Because of the lack time, we decided to perform patch tests among allergic patients using "as is" dental materials such as cements and resins. 65 patients were tested with "as is" materials and 24 patients were tested with single reagents. 10 control non-allergic volunteers were tested with "as is" materials as well. As a result, there were significant numbers of patch test positives among the allergic patients and even a healthy volunteer showed severe positive a resin. We have to study these non-metallic dental material allergies from now on.2.To study genetic differences between allergic patients and healthy individuals, we collected peripheral bloods out of 5 volunteers and proteomes were compared. As a result, we found approximately 10 putative proteins that might be playing some roles in the allergic reactions in the patients.

本研究的目的是建立用于斑贴试验的口腔材料非金属试剂，并研究其生物学反应。1.首先，我们对几种口腔材料的成分进行了分析。然而，我们在6个月左右的时间里未能做到这一点。由于时间不够，我们决定在过敏患者中使用“原样”牙科材料（如粘固剂和树脂）进行斑贴试验。65例患者使用“原样”材料进行检测，24例患者使用单一试剂进行检测。10名对照非过敏志愿者也用“原样”材料进行测试。结果，在过敏患者中有大量斑贴试验阳性，甚至一名健康志愿者也显示出严重的阳性a树脂。2.为了研究口腔非金属材料过敏症患者与健康人的遗传差异，我们收集了5名志愿者的外周血，并进行了蛋白质组比较。结果，我们发现了大约10种可能在患者过敏反应中发挥作用的推定蛋白质。