非金属歯科材料アレルギーを対象としたパッチテスト用試薬開発とその基礎に関する研究

非金属牙科材料过敏斑贴试验试剂的研制及基础研究

• 批准号: 13557164
• 负责人: UOSHIMA Katsumi
• 金额: $ 3.84万
• 依托单位: NIIGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13557164/
• 关键词: non-metallic; patch test; proteomics; allergy; 抗原性

The purpose of this study was to establish non-metallic reagents of dental materials for patch tests and to investigate the biological responses against those antigens.1.First, we tried to analyze the constituents of some specific dental materials. However, we failed to do so after 6 month or so. Because of the lack time, we decided to perform patch tests among allergic patients using "as is" dental materials such as cements and resins. 65 patients were tested with "as is" materials and 24 patients were tested with single reagents. 10 control non-allergic volunteers were tested with "as is" materials as well. As a result, there were significant numbers of patch test positives among the allergic patients and even a healthy volunteer showed severe positive a resin. We have to study these non-metallic dental material allergies from now on.2.To study genetic differences between allergic patients and healthy individuals, we collected peripheral bloods out of 5 volunteers and proteomes were compared. As a result, we found approximately 10 putative proteins that might be playing some roles in the allergic reactions in the patients.

本研究的目的是建立用于斑贴试验的非金属牙科材料试剂，并研究其对这些抗原剂的生物学反应。1.首先，我们试图分析一些特定的牙科材料的化学成分。然而，我们在6个月左右的时间里没有做到这一点。由于时间紧迫，我们决定在过敏患者中进行斑贴测试，使用“原样”的牙科材料，如水泥和树脂。65例患者用“原样”材料检测，24例患者用单一试剂检测。10名对照的非过敏性志愿者也用“原样”材料进行测试。结果，在过敏患者中有相当数量的斑贴试验阳性，甚至健康志愿者的树脂也呈严重阳性。2.为了研究过敏患者和健康人之间的遗传差异，我们采集了5名志愿者的外周血，并比较了蛋白质组。结果，我们发现了大约10种可能在患者过敏反应中发挥作用的假定蛋白质。