非金属歯科材料アレルギーを対象としたパッチテスト用試薬開発とその基礎に関する研究

非金属牙科材料过敏斑贴试验试剂的研制及基础研究

• 批准号: 13557164
• 负责人: UOSHIMA Katsumi
• 金额: $ 3.84万
• 依托单位: NIIGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13557164/
• 关键词: non-metallic; patch test; proteomics; allergy; 抗原性

The purpose of this study was to establish non-metallic reagents of dental materials for patch tests and to investigate the biological responses against those antigens.1.First, we tried to analyze the constituents of some specific dental materials. However, we failed to do so after 6 month or so. Because of the lack time, we decided to perform patch tests among allergic patients using "as is" dental materials such as cements and resins. 65 patients were tested with "as is" materials and 24 patients were tested with single reagents. 10 control non-allergic volunteers were tested with "as is" materials as well. As a result, there were significant numbers of patch test positives among the allergic patients and even a healthy volunteer showed severe positive a resin. We have to study these non-metallic dental material allergies from now on.2.To study genetic differences between allergic patients and healthy individuals, we collected peripheral bloods out of 5 volunteers and proteomes were compared. As a result, we found approximately 10 putative proteins that might be playing some roles in the allergic reactions in the patients.

本研究的目的是建立用于斑贴试验的牙科材料非金属试剂并研究针对这些抗原的生物反应。1.首先，我们尝试分析一些特定牙科材料的成分。然而，大约六个月后，我们未能做到这一点。由于缺乏时间，我们决定使用“原样”牙科材料（例如水泥和树脂）在过敏患者中进行斑贴测试。 65 名患者使用“原样”材料进行测试，24 名患者使用单一试剂进行测试。 10 名对照非过敏志愿者也使用“原样”材料进行了测试。结果，过敏患者中有大量斑贴试验呈阳性，甚至健康志愿者的树脂也呈严重阳性。我们从现在起必须研究这些非金属牙科材料过敏。2.为了研究过敏患者和健康个体之间的遗传差异，我们采集了5名志愿者的外周血并进行了蛋白质组比较。结果，我们发现了大约 10 种可能在患者过敏反应中发挥一定作用的推定蛋白质。