Experimental study on pathogenesis of asthma like symptom caused by combination of diesel exhaust and allergen.

We investigated the histopahologic changes in the airway by long term exposure to diesel exhaust(de),ovalbumin (OA) treatment or the both combination. the relation between the histopahologicappearances in the airway and immunoglobulin production or levels of local cytokines in the lungsICR mice were exposed to clean air or DE at a soot concentrations of 0.3，1.0或3.0 mg/m D13 D1 for 34或40 weeks. Fifteen weeks after exposures to DE，mice were sensitized intraperitonealy with 10 μg of OA，and challenged aerosol of 1% OA at 3-week intervals during the last 18 or 24 weeks of the exposures。Exposure to DE for 34 weeks caused dose-dependent increases of the type changes such asnon-ciliated cell proliferation and epithelial cell hypertrophy in the airway，but showed no effect on goblet cell proliferation in the bronchial epithelium and eosinophilOA challenge induced very alight changes in goblet cell proliferation andeosinophil rec…More ruitment. The combination of OA and DE exposure at 3.0 mg/m . D13 . D1 produced increasedchanges of goblet cells and eosinophils，“Exposure to DE at 3.0 ”Exposure to DE at 3.0 "Exposure to DE at 3.0mg/m D13 D1 for 40 weeks also enhanced allergen-induced eosinophil recruitment into the submucosin the airways，and increased protein levels of GM-CSF and IL-5 in the lungs. the increases in the eosinophilrecruitment and the local cytokine expression were accompanied by those in goblet cell proliferation在bronchial epithelium和airway hypersponsiveness to inhaled acetylcholine. OA treatmentinduced OA-specific IgG1 and IgE production in plasma，whereas the adjutant effects of DE exposure on the immunoglobulin production were thepresent研究提供experimental evidence that daily inhalation of DE can enhance theallergen-induced respiratory disease such as allergic asthma. This effect may be mediated mainly bythe enhanced local expression of IL-5 and GM-CSF. Less

We investigated the histopahologic changes in the airway by long term exposure to diesel exhaust (DE), ovalbumin (OA) treatment or the both combination. The relation between the histopahologic appearances in the airway and immunoglobulin production or levels of local cytokines in the lungs was also studies. ICR mice were exposed to clean air or DE at a soot concentrations of 0.3, 1.0 or 3.0 mg/mィイD13ィエD1 for 34 or 40 weeks. Fifteen weeks after exposures to DE, mice were sensitized intraperitonealy with 10 μg of OA, and challenged aerosol of 1% OA at 3-week intervals during the last 18 or 24 weeks of the exposures. Exposure to DE for 34 weeks caused dose-dependent increases of the typical changes such as non-ciliated cell proliferation and epithelial cell hypertrophy in the airway, but showed no effect on goblet cell proliferation in the bronchial epithelium and eosinophil recruitment in the airway. OA challenge induced very alight changes in goblet cell proliferation and eosinophil rec … More ruitment. The combination of OA and DE exposure at 3.0 mg/mィイD13ィエD1 produced increased changes of goblet cells and eosinophils, in addition to further increases of the typical changes induced by DE. "Exposure to DE at 3.0 mg/mィイD13ィエD1 for 40 weeks also enhanced allergen-induced eosinophil recruitment into the submucos in the airways, and increased protein levels of GM-CSF and IL-5 in the lungs. The increases in the eosinophil recruitment and the local cytokine expression were accompanied by those in goblet cell proliferation in the bronchial epithelium and airway hyperresponsiveness to inhaled acetylcholine. OA treatment induced OA-specific IgG1 and IgE production in plasma, whereas the adjutant effects of DE exposure on the immunoglobulin production were not observed. The present study provides experimental evidence that daily inhalation of DE can enhance the allergen-induced respiratory disease such as allergic asthma. This effect may be mediated mainly by the enhanced local expression of IL-5 and GM-CSF. Less