Community analysis of microorganisms metabolizing multiple aromatic compounds in soil

本研究是如何开发方法来衡量的（1）微生物群降解的芳香化合物的概况， （2）微生物群代谢在土壤中使用的单个基础结构分析（3）微生物群代谢D114微生物群代谢多个基础结构分析（2）微生物群代谢多个基础结构分析（2）微生物群使用的微生物群D114微生物群异构结构分析（2）微生物群代谢多个基础结构分析）。（1） The profile of microbial population degrading aromatic compounds were determined by using indicator of dehydrogenase activity of microorganisms degrading aromatic compounds and by coloring reaction of chloride anion released from chloroaromatic compounds.该结果提出了一项关于农场管理在土壤中的应用的结果，即增加土壤中的微生物种群，使土壤中的降解芳香化合物。人口退化， 2，4-二氯酚在土壤中的应用是由农场占用的，但不是3-氯酚。（2） Using glucose， glycine and acetate，D114-D1C quinone profile method被开发用于检测微生物群降解一个单一的基础。在土壤测试中，对活跃微生物群落的统计分析的可再生性是如此之高。（3） Phenol and benzoate were used as model aromatic compounds。D114-D1C quinone profile method被用作model aromatic metabolization phenol in the presence of benzoate and vice versa.芳香化合物的矿物化比率和总的quinone概况和标签的quinones在重复的实验中几乎是唯一的，但在D114的quinone概况完全不同。本文建议土壤中的微环境影响了土壤中的微环境，使土壤中的芳香化合物变化。

This study conducted to develop the methods to measure (1) the profile of microbial population degrading aromatic compounds i soil, (2) the microbial groups metabolizing a single substrate in soil using ィイD114ィエD1C quinone profile analysis and (3) the microbial groups metabolizing multiple substrates in soil using ィイD114ィエD1C quinone profile analysis. (1) The profile of microbial population degrading aromatic compounds were determined by using indicator of dehydrogenase activity of microorganisms degrading aromatic compounds and by coloring reaction of chloride anion released from chloroaromatic compounds. The results suggested that application of farmyard manure into soil increase the microbial population to degrade aromatic compounds in soil. The population degrading, 2,4-dichlorophenol in soil was increased by application of farmyard manure but not that degrading 3-chlorophenol. (2) Using glucose, glycine and acetate, ィイD114ィエD1C quinone profile method was developed to detect the microbial groups degrading a single substrate. The reproducibility was high so that the statistical analysis of active microbial community between the soils examined. (3) Phenol and benzoate were used as model aromatic compounds. The ィイD114ィエD1C quinone profile method was used as model aromatic metabolizing phenol in the presence of benzoate and vice versa. Mineralization rates of aromatic compounds and total quinone profiles and labeling rates of quinones were almost identical between the duplicated experiment but the ィイD114ィエD1C quinone profiles were completely different. This suggested that the microenvironment in soil affected the microbial groups metabolizing aromatic compounds in soil.