Screening for signal-responsive genes by an enhancer-trap vector and their application

作为使用逆转录病毒载体（pROSA-nGBT）的新开发的增强子捕获系统的应用，我们筛选了对TGF-β或博来霉素应答的基因，成功地分离了每种情况下的多个感兴趣的基因，并且可以发表这些结果的一部分。其中一个TGF-β-应答基因被发现编码一种新的位于内质网（ER）中的硫氧还蛋白相关跨膜蛋白，并被认为是参与ER应激管理的分子。其中一个对博莱霉素（一种诱导DNA双链断裂的药物）有反应的基因被鉴定为已知参与细胞凋亡的C-IAP 2。由于该基因的启动子也被发现对治疗剂量的电离辐射有反应，因此它在辐射基因治疗中的应用是一个有趣的可能性，值得进一步研究。

As applications of the newly developed enhancer trap system using a retroviral vector (pROSA-nGBT), we screened genes responsive to TGF-β or bleomycin, succeeded in isolating multiple genes of interest in each case, and could publish a part of these results. One of the TGF-β-responsive genes was found to encode a novel, thioredoxin-related transmembrane protein localized in the endoplasmic reticulum (ER) and was suggested to be a molecule involved in the management of ER stress. One of the genes responsive to bleomycin, a drug inducing DNA double strand break, was identified as C-IAP2 know to be involved in apoptosis. Since the promoter of this gene was found also to respond to therapeutic dosages of ionizing radiation, its application in radiation gene therapy is an interesting possibility and warrants further investigation.