Novel recognition methods for environmental hormones based on spatial multi-points interaction

生孔分子印迹效应生孔分子印迹效应可以利用与真实目标分子具有相似结构的模板分子。详细的研究表明，通过致孔分子印迹效应制备的聚合物往往能够识别传统分子印迹效应难以识别的相对较大的分子。通常情况下，环境激素具有较大的结构体积，因此成孔剂的印迹效应对于识别这些化合物是非常有用的。传统的以乙烯基吡啶或乙烯基咪唑为功能单体的分子印迹技术可以作为模板分子，而叔丁基苯酚也可以作为双酚A的模板分子。与双酚A模板分子相比，以叔丁基苯酚为模板分子制备的聚合物对双酚A的选择性更高。所获得的吸附能力甚至可以与活性碳相媲美。新的分离介质及其性能我们利用新的膜乳化技术以及多孔整体式分离介质(棒柱)制备了尺寸均匀的聚合物颗粒，以考察其分离性能以及作为我们所用的吸附剂的可能性。因此，粒径的均匀性对于环境水溶液的处理是非常重要的。此外，棒状柱在毛细管电色谱仪中提供了可能超高柱效

Porogen imprinting effectPorogen imprinting effect can utilize the template molecule having similar structure to the real target molecule. The detail study revels that the polymer prepared through porogen imprinting effect tends to recognize relatively bulky molecule that can be hardly recognized through traditional molecular imprinting effect. Usually, environmental hormones has rather structurally bulky structure, therefore the porogen imprinting effect is quite useful for the recognition of those compounds. In addition, the molecule which is a part of larger molecule, such as oligopeptides and/or polymers can be utilized as "Fragment template" to realize selective molecular recognition to large molecules.Molecular imprinting toward Bisphenol ABisphenol A known as an environmental hormone can be utilized as the template molecule for traditional molecular imprinting technique with vinyl pyridine or vinyl imidazole as a functional monomer, but t-butyl phenol can be also utilized as a template molecule toward bisphenol A.In fact, those polymer prepared using t-butyl phenol as template molecule can be afford even higher selectivity to bisphenol A compared with those prepared bisphenol A as the template molecule. The obtained absorption ability is even comparable to that of activated carbons.New separation media and those propertiesWe have prepared uniformly sized polymer particles using new membrane emulsification technique as well as porous monolith type separation media (rod column) to investigate those separation properties as well as possibility as absorbents for our purpose. In the result, size uniformity is quite important for the treatment of environmental aqueous solution. In addition, rod column afford possible ultra high column efficiency in capillary electorochromatograh