Group recognition for Natural Toxic Compounds based on Their Toxicity and Its Contribution to Rapid Analysis

1)新型片段印迹方法的研究对于天然有毒化合物微囊藻毒素，采用ADDA官能团作为伪模板分子的新型片段印迹方法。有趣的是，即使烷基苯类化合物也可以作为微囊藻毒素的有效假模板，此外，基于烷基苯类印迹聚合物的分子识别能力相对较弱，可以实现对微囊藻毒素的有效基团识别。这种识别基于它们的毒性识别。2)基于毒性识别的基团识别除了上述结果之外，谷氨酸部分的印迹对于基团识别也是非常有效的。3)环境水的前处理所得到的分离介质显示出目标同系物的浓缩效应，而其他污染物很容易从分离介质中被排除。这是非常重要的，因为使用所获得的分离介质可以更容易地进行前处理。

The following results have been obtained along 2 years' project.1)Development of novel fragment imprinting methodFor a naturally occurring toxic compound, Microcystin, the novel fragment imprinting method was applied using Adda functional group as pseudo-template molecule. Interestingly, even alkylbenzenes could be utilized as effective pseudo-template to Microcystins, in addition, relatively loose molecular recognition ability based on alkylbenzene imprinted polymer was found to realize effective group recognition of Microcystins. This recognition was found to be based on their toxicity recognition.2)Group recognition based on toxicity recognitionIn addition to the results described above, an imprinting for glutamic acid moiety was also quite effective for the group recognition. This was found to be based on interesting exclusion mechanism for other less important functional groups of Microcystins.3)Pre-treatment of environmental waterthe separation media obtained showed concentration effect of the target homologues, while other contaminants were easily excluded from the separation media. This is very important because much easier pre-treatment can be done using the obtained separation media.