Group recognition for Natural Toxic Compounds based on Their Toxicity and Its Contribution to Rapid Analysis

基于天然有毒化合物的毒性及其对快速分析的贡献的群体识别

• 批准号: 13640604
• 负责人: HOSOYA Ken
• 金额: $ 2.24万
• 依托单位: Kyoto Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13640604/
• 关键词: Fragment Imprinting Method; Artificial Receptor; Mycrocystins; Pre-treatment; HPLC; Molecular Imprinting; Toxicity Recognition; Nano-technology; 人工レセプター; ナノテクノロジ

The following results have been obtained along 2 years' project.1)Development of novel fragment imprinting methodFor a naturally occurring toxic compound, Microcystin, the novel fragment imprinting method was applied using Adda functional group as pseudo-template molecule. Interestingly, even alkylbenzenes could be utilized as effective pseudo-template to Microcystins, in addition, relatively loose molecular recognition ability based on alkylbenzene imprinted polymer was found to realize effective group recognition of Microcystins. This recognition was found to be based on their toxicity recognition.2)Group recognition based on toxicity recognitionIn addition to the results described above, an imprinting for glutamic acid moiety was also quite effective for the group recognition. This was found to be based on interesting exclusion mechanism for other less important functional groups of Microcystins.3)Pre-treatment of environmental waterthe separation media obtained showed concentration effect of the target homologues, while other contaminants were easily excluded from the separation media. This is very important because much easier pre-treatment can be done using the obtained separation media.

经过两年的研究，取得了以下成果：1)新型片段印迹方法的开发针对天然存在的有毒化合物微囊藻毒素，以Adda官能团作为伪模板分子，应用了新型片段印迹方法。有趣的是，甚至烷基苯也可以作为微囊藻毒素的有效假模板，此外，基于烷基苯印迹聚合物的相对松散的分子识别能力也可以实现对微囊藻毒素的有效基团识别。发现这种识别是基于它们的毒性识别。2)基于毒性识别的基团识别除了上述结果之外，谷氨酸部分的印迹对于基团识别也非常有效。这被发现是基于对微囊藻毒素其他不太重要的官能团的有趣排除机制。3)环境水的预处理获得的分离介质显示出目标同系物的浓缩效应，而其他污染物很容易从分离介质中排除。这非常重要，因为使用获得的分离介质可以更容易地进行预处理。

项目成果

Ken Hosoya他: "Novel surface modification of polymer-based separation media controlling separation selectivity, retentivity and generation of electroosmotic flow"Journal of Chromatography A. 979. 3-10 (2002)

Ken Hosoya 等人：“控制分离选择性、保留性和电渗流产生的聚合物分离介质的新型表面改性”《色谱杂志》A. 979. 3-10 (2002)

Ken Hosoya: "A possible purification method of DNAs' fragments from humic matters in soil extracts using novel stimulus responsive polymer adsorbent"J. Pharm. and Biomed. Anal.. 30. 1919-1922 (2003)

Ken Hosoya：“使用新型刺激响应聚合物吸附剂从土壤提取物中的腐殖质中纯化 DNA 片段的可能方法”J.

Ken Hosoya他: "Preparation strategy for uniformly sized, polymer-based HPLC packing materials having practically acceptable column efficiency. 1. Copolymerization technique"Analytical Chemistry. 73・24. 5852-5857 (2001)

Ken Hosoya 等人：“具有实际可接受的柱效率的均匀尺寸的聚合物基 HPLC 填充材料的制备策略。1. 共聚技术”分析化学 73・24（2001 年）。

Ken Hosoya他: "Capillary Electrochromatography on Monolithic Silica Columns"Analytical Science. 18. 89-92 (2002)

Ken Hosoya 等人：“整体二氧化硅柱上的毛细管电色谱”分析科学。18. 89-92 (2002)

Ken Hosoya: "Preparation strategy for uniformly sized, polymer -based HPLC packing,materials having practically acceptable colum n effciency. 1. Copolymerization technique"Analytical Chemistry. 73・24. 5852-5857 (2001)

Ken Hosoya：“均匀尺寸的聚合物基 HPLC 填料的制备策略，具有实际可接受的柱效率。1. 共聚技术”分析化学 73・24（2001）。