Development of munti-functional and dimensional HPLC stationary phases using molecular imprinting.

为了开发多维固定相，本文对分子印迹技术和选择性表面修饰方法进行了研究。在此基础上，将分子印迹技术与表面修饰方法简单地结合起来，就可以制备出目标固定相。为了进一步研究，本文制备了多种分子印迹固定相，并从化学和液相色谱的角度对分子印迹固定相的孔结构进行了研究。此外，我还应用了新型的温度响应性外层或疏水性外层从复杂混合物中分离目标部分，得到了以下结果.首次报道了致孔剂印迹效应。致孔剂印迹实现了对结构相似化合物的分子识别.致孔剂印迹也提供了优先保留对更有毒的化合物，如共面PCB。采用亲水性外层选择性修饰的分子印迹固定相实现了直接进样和分析。

For the development of multi-dimensional stationary phases, I studied molecular imprinting technique and selective surface modification method. On the basis of the above results, the objective stationary phases can be prepared a simple combination of molecular imprinting technique and surface modification method.For the further study, I prepared a variety of molecularly imprinted stationary phases and studied porous structures from the viewpoints of chemistry as well as liquid chromatog-raphy. In addition, I applied novel temperature-responsive external layer or hydrophulic ex-eternal layer for separation of the target moiety from complicated mixtures.Finally, I obtained the following results.1. Porogen imprinting effects was firstly reported. Porogen imprinting realized molecular recognition towards structurally similar compounds.2. Porogen imprinting also afforded preferential retention towards more toxic compounds such as coplanar PCBs.3. Direct injection and analysis were realized using molecularly imprinted stationary phases selectively modified with hydrophilic external layer.