Studies on super sensitive analysis with atto mole order by means of capillary electrophoresis equipped with chemiluminescence detection. -Development of high performance chemiluminescence detection cell using an optical fiber-

设计了一种新型、简便的光纤化学发光毛细管电泳检测池。该细胞易于与毛细管电泳相结合，操作简便。鲁米诺化学发光适用于该检测细胞。详细考察了过氧化氢和催化剂在电池中的使用和浓度。鲁米诺的检出限为5.0 × 10^<-10> M (14 amol; S/N = 3)，是目前报道的灵敏度最高的结果。另外，用异硫氰酸异氨基醇标记的甘氨酸、甘氨酸和甘氨酸的混合物样品也适用于本体系。它们被灵敏地检测到，并以完全的基线分离表示。利用光纤化学发光检测池的概念进行了以下研究：《毛细管等电聚焦分离血红素蛋白的化学发光检测》、《过氧草酸盐化学发光毛细管电泳成批型检测池》、《毛细管等电聚焦-化学发光检测压力动员系统的设计》、《光纤毛细管电泳流动型化学发光检测池》。

A new and simple chemiluminescence detection cell using optical fiber was designed for capillary electrophoresis. The cell was easily combined with capillary electrophoresis and the system was operated without any tedious procedures. Luminol chemiluminescence was adapted for use with this detection cell. Hydrogen peroxide and catalysts were in detail examined concerning their usage and concentration with the cell. The detection limit for luminol was 5.0 x 10^<-10> M (14 amol ; S/N = 3), which was the most sensitive result reported so far. Also, a mixture sample of glycine, glycylglycine, and glycylglycylglycine which were labeled with isoluminol isothiocyanate was subjected to the present system. They were sensitively detected and represented with complete base-line separations.The concept of chemiluminescence detection cell using optical fiber was applied for the following researches : "Chemiluminescence detection of heme proteins separated by capillary isoelectric focusing", "Batch-type detection cell using a peroxyoxalate chemiluminescence system for capillary electrophoresis", "Design of pressure-mobilization system for capillary isoelectric focusing - chemiluminescence detection", and "Flow-type chemiluminescence detection cell using optical fiber for capillary electrophoresis".