Effects of Cyclophosphamide and Dioxin (TCDD) on spermatogenesis in rats

环磷酰胺和二恶英对大鼠精子发生的影响[j]。GH对环磷酰胺（CP）致睾丸损伤大鼠恢复的影响。CP以50 mg/kg的剂量静脉给药，连续2天；GH以0.3 mg/kg的剂量皮下给药，连续2周和4周。A型精原细胞暴露于CP后，精母细胞数量减少。这个。CP/GH组可使精子活力指数降低，恢复精子活力指数，改善精子活力指数。在GH缺乏的情况下，外源性GH可以改善cp损伤的睾丸功能。母体大鼠给予极低剂量TCDD对新生大鼠性腺功能的影响。母鼠（出生后7、14、21天和7、14天）以10 Rg/kg的剂量皮下注射TCDD，每周1次。新生大鼠出生后21天。各脏器肉眼检查正常，睾丸组织镜下检查正常。TCDD特异性诱导的Cyp lalp在新生大鼠肝脏中的表达明显增加。睾丸组织中抑制素B（支持细胞功能标志物）表达正常。

Effects of Cyclophosphamide and Dioxin (TCDD) on spermatogenesis in rats1. Effects of GH on recovery from testicular damage induced by Cyclophosphamide (CP) in GH-deficient rats. CP was intravenously administered at dose of 50 mg/kg for 2 days, and rat GH was subcutaneously administered at dose of 0.3 mg/kg every day for 2 and 4 weeks. The number of spermatocytes was decreased when they exposed to CP at type A spermatogonia. The.motility index of spermatozoa was also decreased by CP. In CP/GH group, the number of those cells was recovered and the damaged motility of spermatozoa was improved. Under GH-deficient condition, exogeneous GH can improve the testicular function damaged by CP.2. Effect of very low dose of TCDD administered to maternal rats on ganadal function of neonatal rats. TCDD was subcutaneously administered at dose of 10 Rg/kg once a week to maternal rats (7, 14, 21 dpc, and 7, 14 days after birth). 21days after birth neonatal rats were subjected. All organs were normal by macroscopic examination, and testicular tissues were also microscopically normal. Expression of Cyp lAlspecifically induced by TCDD was markedly increased in neonatal rat liver. Inhibin B (marker of Sertoli cell function) expression in testis was normal.