The clarification of pathology of allergic Reactions to gelatin-containing vaccine.

我们用明胶体外二次刺激循环单核细胞，研究了对含明胶疫苗过敏患者T淋巴细胞的特征。立即反应的患者都有明胶特异性IgE抗体，而其他人则没有。非立即型反应患者的淋巴细胞通常表达非常弱或有时不表达IFN-γ、IL-2或IL-13基因，特别是不表达IL-4基因。另一方面，即时型反应受试者的淋巴细胞不仅显著表达IL-4和IL-13，而且显著表达IFN-γ和IL-2 mRNA。这表明明胶特异性Th2和Th1反应都参与了对明胶的直接反应的发病机制。在立即反应的患者中一致观察到的明胶特异性IL-4和/或IL-13反应可能与明胶特异性IgE抗体的产生有关。

We investigated the characteristics of T lymphocytes from patients allergic to gelatin-containing vaccine by secondary in vitro stimulation of circulating mononuclear cells with gelatin.Patients with an immediate reaction all had gelatin-specific IgE antibody, whereas others did not. Lymphocytes from subjects with nonimmediate-type reactions generally expressed very weak or sometimes no IFN-γ, IL-2 , or IL-13 genes and especially no IL-4 gene. On the other hand, the lymphocytes of subjects with immediate-type reactions significantly expressed not only IL-4 and IL-13 but also IFN-γ and IL-2 mRNA.These suggest that both gelatin-specific Th2 and Th1 responses are involved in the pathogenesis of the immediate reaction to gelatin. The gelatin-specific IL-4 and/or IL-13 responses consistently observed in patients with an immediate reaction may be associated with the production of gelatin-specific IgE antibody.