Ultra High-Performance Liquid Chromatography High-Resolution Mass Spectrometer (UHPLC-HRMS)

我们申请超高效液相色谱高分辨率质谱仪用于微生物和微生物群落的代谢组学研究。该仪器将主要用于监测微生物对群落内不同相互作用伙伴的代谢反应，并识别调节群落结构的信号代谢物。利用现代质谱法的力量，将对实验室实验的模型群落以及来自中观和野外工作的样本进行调查。该仪器将为CRCs ChemBioSys和AquaDiva组织的几个小组提供平台，并将成为EXC Balance of The Microverse仪器园区的一部分。代谢组学是一项成熟的技术，可以监测给定生物系统内的代谢变化。它作为一种假设生成的方法，通过识别受外部因素（如压力或互动伙伴的存在）调节的代谢途径。借助强大的化学信息学支持，可以挖掘作为候选化学介质的代谢物的大型数据集。特别是现代质谱仪的高分辨率和快速扫描速率允许对代谢物进行全面表征。最新一代仪器的灵敏度一方面将允许在复杂的自然样品中处理甚至痕量化合物，但也允许小型化和监测样品，例如，从微流体实验中产生的样品。由此产生的质谱数据也将为计算机辅助结构解析的新化学信息学方法的发展奠定基础。一方面，该仪器将促进分析化学方法的研究，并支持生物有机分析部门的活动。另一方面，它还将使生物科学学院、化学和地球科学学院以及数学和信息学学院的更大联盟内的合作项目成为可能。因此，该装置将在耶拿遗址作为众多研究活动的技术平台发挥重要作用。

We apply for an Ultra High-Performance Liquid Chromatography High-Resolution Mass Spectrometer for the metabolomic investigation of microorganisms and microbial communities. The instrument will mainly serve to monitor the metabolic responses of microorganisms to different interaction partners within communities and to identify signaling metabolites that regulate the community structure. Model communities from laboratory experiments as well as samples from mesocosm and fieldwork will be investigated by exploiting the power of modern mass spectrometry. The instrument will contribute to a platform for several groups organized within the CRCs ChemBioSys and AquaDiva and it will be part of the instrument park of the EXC Balance of the Microverse. Metabolomics is a mature technique that allows the monitoring of metabolic changes within given biological systems. It serves as a method for hypothesis generation by identification of metabolic pathways that are regulated in response to external factors, such as stress or the presence of interaction partners. With powerful chemoinformatic support, large datasets can be mined for metabolites that are candidate chemical mediators. Especially the high resolution of modern mass spectrometers and the fast scan rate allows for the comprehensive characterization of metabolites. The sensitivity of the newest instrument generation will allow, on the one hand, to address even trace compounds within complex natural samples but also enable miniaturization and monitoring of samples generated, e.g., from microfluidics experiments. The resulting mass spectrometric data will also form the basis for the development of new chemoinformatics approaches for computer-aided structure elucidation. On the one hand, this instrument will thus advance research in method-developing analytical chemistry and support activities in the department of bioorganic analytics. On the other hand, it will also enable collaborative projects within larger consortia within the faculties of biosciences, faculty of chemistry and earth sciences and the faculty for mathematics and informatics. The device will thus have an important function at the Jena site as a platform technology for numerous research activities.