Molecular Cloning of New Sodium Chloride Transporters

该项目的目的是尽可能多地确定转运钠和/或氯的转运蛋白和通道，以便我们能够更清楚地了解我们体内的水和电解质代谢。由于水的运输依赖于NaCl的渗透驱动，因此也寻找水通道（水通道蛋白）。随着基因组计划的出现，新基因的克隆过程得到了极大的便利。利用这一点，一些新的基因被克隆和组织分布进行了研究。其中部分基因已在非洲爪蟾卵母细胞表达系统中表达。三个新的阳离子-氯离子-协同转运蛋白（CCC 6、CCC 7、CCC 8）、一个新的钠-磷酸盐协同转运蛋白、一个新的阴离子交换蛋白、两个新的氯离子/硫酸盐交换蛋白、一个新的阳离子通道（双孔通道1 TPC 1）、8个新的四跨膜通道、两个新的辣椒素受体样通道、两个新的水通道蛋白样氯离子通道、一个新的钠-氯离子-儿茶酚胺转运蛋白和一个新的葡萄糖转运蛋白。这些膜蛋白的进一步表征需要与特定领域的专家合作。

The aim of this project was to identify transporters and channels that transport sodium and/or chloride as many as possible so that we can get the clearer view on water and electrolyte metabolism in our bodies. As the transport of water is depend on the osmotic drive by NaCl, water channels (aquaporins) were also searched for. With the advent of genome projects, the process of cloning of new genes was extremely facilitated. Taking advantage of this, several new genes were cloned and the tissue distributions were examined. Some of them were functionally characterized with Xenopus oocyte expression system. Three new Cation-Chloride-Cotransporters (CCC6, CCC7, CCC8), a new sodium-phosphatecotransporter, a new anion exchanger, two new chloride/sulfate exchangers, a novel form of cation channel (Two-Pore-Channel 1 TPC1), 8 new tetraspanning channels, two new capsaicin receptor-like channcls, two new aquaporin-like chloride channels, a new sodium-chloride-catecholamine transporter and a new glucose transporter. Further characterization of thesc membrane proteins are necessary with collaborative works together with experts in the specific fields.