The research of Electrochemotherapy and bone electroconductivity of the mirine osteosarcoma model of bone lesion.

电疗可增强抗肿瘤药物进入骨病变的小鼠骨肉瘤细胞。在这个实验模型中，我们研究了电疗的疗效和原因是否与骨的电导率、电极之间的距离以及电极的配置和数量的差异有关。材料与方法将切除的人骨肉瘤切成细块，植入SCID小鼠转成的裸鼠后肢骨髓中，在骨内形成实体鼠肿瘤。移植后通过x线摄影检查肿瘤直径，当肿瘤直径达到5mm时开始实验。采用BTX的电穿孔器（T820）进行电穿孔。依次比较药物组（D组）+电穿孔组（E组）和不用电穿孔组（D组和E组）肿瘤体积的变化。作为抗肿瘤药物，我们使用博来霉素（BLM）。测量了固定肿瘤的n个电极之间的电阻率，并计算了电穿孔产生的热量（卡）。采用生物测定法对BLM肿瘤组织浓度进行分析，并比较D+E组和D不加E组的肿瘤组织浓度。结果与讨论电穿孔组肿瘤直径明显减小。电极间距为10mm、15mm、20mm和间隔的组肿瘤直径减小。有效例为38肢中的23肢。多剂量组手术时有效例数增加，比较1次手术、2次手术、3次手术的频率（D+E+）。肿瘤组织中BLM的浓度较高，因为在抗癌药物中无电穿孔组（D+E-）与电穿孔组（D+E+）比较，BLM在肿瘤组织中的浓度较高，似乎是电穿孔的作用使BLM掺入骨间肿瘤组织。由肿瘤间电阻率计算的产热对抗肿瘤效果没有影响。在x线照片显示骨破坏恢复进展的例子中，病理组织检索中有肿瘤细胞坏死和消除。这似乎是证明经骨电疗骨间病变具有细胞毒性反应的观点。电极之间的距离和效应之间的意义是不可接受的。少

INTRODUCTIONElectrochemotherapy is available to enhance the entry of anti-neoplastic agents into murine osteosarcoma cells in the bone lesion. In this experiment model, we investigated the efficacy and cause of electrochemotherapy whether it attributed to the electroconductivity of bone, the distance between electrode and the difference between configuration and number of electrode.MATERIALS AND METHODSTo produce solid murine tumors in bone, resected human osteosarcoma was cut into fine pieces, and inserted into the bone marrow of the hind-limb of Nude rat converting from the SCID mouse.After transplantation, the tumor diameter was checked by X-ray photogram and the experiment was started when it reached 5 mm. The electroporation was done by the electroporator (T820) of BTX.The changes of tumor volume were compared sequentially among the two groups : Drug (D) + Electroporation (E) and neither D nor E.As an anti-neoplastic agent, we used bleomycin (BLM).The electrical resistivity betwee … More n electrodes which held the tumor was measured, and heat (Calaory) which arises by electroporation was calculated.The tumor tissue concentration of BLM was analyzed based on the bio-assay and compared among the two groups : D+E and D without E.Histology was investigated on the tumor necrosis.RESULTS AND DISCUSSIONSThere was the reduction of the tumor diameter in the electroporation (E) group. The reduction of the tumor diameter has been obtained in the group in which the distance between electrodes spread 10mm, 15mm, 20mm and interval. Effective examples were the 23 limbs in 38 limbs.The effective example increased in multiple dose group under operation, when it was compared at the frequency in which 1 time operation, 2 time operation, 3 time operation did (D+E+).The concentration in the tumor tissue of BLM showed the high value, since it has it, in comparing without electroporation group (D+E-) in anti-cancer drug and electroporation group (D+E+), and it seemed to be the effect of electroporation that BLM was incorporated in the interosseous tumor tissue. The production heat calculated from electrical resistivity between tumors did not bring about the effect in anti-tumor effect. In-the example of showing the progress that the bone destruction was restored in X-ray photogram, there were necrosis and elimination of the tumor cell in the pathohistological retrieval. It seemed to be the opinion which proved that electrochemotherapy for the interosseous lesion through the bone has the cytotoxicity reaction.The significance could not be accepted between distance and effect between electrodes. Less