Effects of anesthetic agents on immunological system and cytokine netorks

1. 挥发性麻醉剂对PBMC mRNA表达的影响(1)本研究纳入口腔手术患者。我们在切口后一小时取血，改变挥发性麻醉剂如异氟醚和七氟醚的浓度。结果表明，两组间差异不显著，但挥发性麻醉药浓度越高，mRNA表达受抑制程度越高。(2)将PBMC漂浮于溶解异氟醚、七氟醚等挥发性麻醉剂的溶液中，研究溶液浓度与PBMC mRNA表达的关系。两组间无显著性差异，但随着麻醉浓度的增加，mRNA的表达受到抑制的程度越高。静脉麻醉药对PBMC mRNA表达的影响。将PBMC漂浮于溶有异丙酚、咪达唑仑、氯胺酮等静脉麻醉药的溶液中，研究溶液浓度与PBMC mRNA表达的关系。异丙酚与咪达唑仑之间无显著性差异，但静脉麻醉药浓度越高，表达越受抑制。相反，随着氯胺酮浓度的升高，mRNA的表达量呈增加趋势。

1. Influence of volatile anesthetics on mRNA expressions of PBMC(1) Oral surgery patients were included in this study. We sampled the blood at an hour after incision, changing the concentration of volatile anesthetics such as isoflurane and sevoflurane. As a result significant difference was not existed between them, but the more concentration of volatile anesthetics increased, the more the mRNA expression was suppressed.(2) After floating PBMC in the solutions dissolved volatile anesthetics such as isoflurane and sevoflurane, we investigated the relationships between concentrations of solutions and mRNA expressions of PBMC.Significant difference was not existed between them, but the more concentration of volatile anesthetics increased, the more the mRNA expression was suppressed.2. Influence of intravenous anesthetics on mRNA expressions of PBMCAfter floating PBMC in the solutions dissolved intravenous anesthetics such as propofol, midazolam, ketamine, we investigated the relationships between concentrations of solutions and mRNA expressions of PBMC.Significant difference was not existed between propofol, midazolam, but the more concentration of intravenous anesthetics increased, the more expression was suppressed. As the concentration of ketamine rose, conversely, the amounts of mRNA expression showed a tendency of increasing.