Expression of adhesion molecules and VEGF in Childhood Malignant Solid Tumors

而血管生成因子可能在神经母细胞瘤的生物学中起重要作用，神经母细胞瘤经常发生血源性扩散。其传播机制尚不清楚。我们从血管生成的角度研究肿瘤的进展和侵袭，试图了解这类肿瘤的特点。我们从1988年至1998年在我院治疗的神经母细胞瘤患者中切除31例标本，用免疫组织化学方法检测VEGF及其受体（Flk-1）的表达。采用抗vegf单克隆抗体和Flk-1多克隆抗体进行染色。采用抗viii因子相关抗原进行微血管检测。我们寻找VEGF及其受体的表达、微血管密度和各种临床病理因素之间的相关性。此外，我们使用原位杂交技术检测了1998年至1999年切除的10例原发性神经母细胞瘤中VEGF mRNA和Flk-1 mRNA的表达和位置。原位杂交和免疫组化均证实神经母细胞瘤细胞内存在VEGF表达。根据原位杂交，我们在神经母细胞瘤细胞中发现了VEGF mRNA，但在血管内皮细胞中没有发现。此外，根据原位杂交，FlK-1 mRNA存在于神经母细胞瘤细胞和血管内皮细胞中。使用Shimada标准的不利组织学中VEGF表达水平高于有利组织学。我们认为旁分泌和自分泌系统参与了神经母细胞瘤的血管生成，VEGF的表达与神经母细胞瘤的预后有关。

While angiogertic factors may play an important role in the biology of neuroblastoma, which frequently spreads hematogenously. the mechanism of spread remains unclear. We studied tumor progression and invasion from the perspective of angiogenesis, and sought to understand the features of this type of tumor. Thirty-one specimens were resected from patient with neuroblastoma treated at our institution from 1988 to 1998 and the expressions of VEGF and its receptor (Flk-1) were examined using immunohistochemistry. Staining was performed using anti-VEGF monoclonal antibody and Flk-1 polyclonal antibody. A microvessel deteccion procedure was performed using anti-Factor VIII-related antigen. We looked for correlations among the expressions of VEGF and its receptors, microvessel density, and various clinicopathologic factors. In addition, we examined the expression and location of VEGF mRNA and Flk-1 mRNA in 10 primary neuroblastomas resected from 1998 to 1999, using in situ hybridizacion. Both in situ hybridization and immunohistochemistry demonstrated the presence of VEGF expression within the neuroblastoma cells. We flound VEGF mRNA in neuroblastoma cells but not vascular endothelial cells, according to in situ hybridization. Further, FlK-1 mRNA is present both in neuroblastoma cells and vascular endothelial cells, according to in situ hybridization. The level of VEGF expression is higher in unfavorable histology using the criteria of Shimada than in favorable histology. We suggested that paracrine and autocrine systems are involved in the antgiogenesis of neuroblastoma and that the expression of VEGF correlates with the prognosis in neuroblastoma.