Defense mechanism of the junctional epithelium in the of periodontal tissue by the adhesive molecule

在牙周病的发展过程中，牙釉质表面上皮附着破坏或脱离是牙周炎初期必需的。同时，新形成的上皮与再生的结合上皮附着在牙表面，成为牙周病愈合阶段不可或缺的因素。为了进一步认识牙周疾病，阐明上皮细胞通过黏附分子和细胞外基质附着在牙釉质表面和暴露根表面的机制是十分重要的。本研究旨在阐明正常大鼠结缔组织上皮和再生大鼠结缔组织上皮中粘附分子和配体细胞外基质的表达。在正常大鼠结膜上皮中，常规免疫荧光染色可在位于牙釉质表面侧的内层基底层识别出Laminin-5阳性反应，呈清晰的细线。更多grin-α6β4是细胞膜上的粘附蛋白之一，是半桥粒的组成部分。层粘连蛋白-5是整合素-α6β4的配体。整合素-α6 β4同样存在于位于牙釉质面侧的内基层中，也存在于位于结缔组织面侧的外基层中。免疫电镜下，采用超薄冷冻切片法的高分辨率免疫胶体金标记法，表明Laminin-5定位的10nm胶体金颗粒仅位于内基底层。这些金颗粒的排列与内部基层的层密度相对应。另一方面，在结膜上皮外基底层只识别出少量与本底水平相同的金颗粒。这些结果强烈提示，在连接上皮中，Laminin-5的表达在内外基底层存在差异，而最内层的连接上皮细胞分泌Laminin-5是上皮附着的关键。对Laminin-5亚基、α3-、β3-、y2链的详细研究有待进一步深入。少

During the progress of periodontal disease, the epithelial attachment destruction or detachment from enamel surface is necessary at the initial stage of marginal peridontitis. In the meantime, the newly formed epithelial attachment to the tooth surface with regencrative junctional epithelium becomes an indispensable factor in during the healing stage of periodonatal disease. For understanding the perriodontal disease, it is important to elucidate the mechanism of epithelial attachment to the enamel surface and exposed root surface with adhesive molecule and extracellular matrix.The purpose of this study is to clarify, the expression of adhesive molecule and the ligand extracellular matrix in the normal rat junctiona epithlium and the regenerative junctional epithelium. In the normal rat junctional epithelium, Laminin-5 positive reaction was recognized at the exclusively inner basal lamina located in enamel surface side as clear thin line by conventional immunofluorescent staining. Inte … More grin-α6β4 is one of the adhesion protein on the cell membrane and component of the half desmosome. Laminin-5 is the ligand of Integrin-α6β4. Integrin-α6 β4 also located in similarly existing in the inner basal lamina located in enamel surfaco side and also, in the outer basal lamina located in connective tissue surface sire. Immunoelectron microscopically, using the high resolution immuno colloidal gold labelling method by ultra thin frozen section methods, 10nm colloidal gold particle which indicated Laminin-5 localization were exclusivoly located in the inner basal lamina. These gold particles were lined correspond to the lamina densa of the inner basal lamina. On the other hand, only few gold particles same as background level were recognized at the outer basal lamina of junctional epithelium. These results strongly suggested that, in the junctional epithelium, Laminin-5 expression is difrerence between the inner and outer basal lamina, and Laminin-5 secretion of the most inner side of the junctional epithelial cells was key of the epithelial attachment. Father study in detail on Laminin-5 sub units, α3-、β3-、y2 chain have to examine in future. Less