Mechanism of salivary secretion and activation of paracellular pathway.

唾液分泌机制和细胞旁通路激活。

• 批准号: 20592152
• 负责人: HASHIMOTO Sadamitsu
• 金额: $ 2.16万
• 依托单位: Tokyo Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20592152/
• 关键词: 唾液腺; 唾液分泌; タイト結合; 細胞骨格; 傍細胞経路; isoproterenol; carbachol; 凍結割断法

To elucidate the morphological change of the paracellular route for fluid secretion, the cytoskeleton of TJs were examined by TEM, using deep etching freeze-fracture (FF) replicas. Isolated submandibular glands (SMGs) were stimulated with 1 μM of carbachol (CCh) and isoproterenol (IPR). After CCh/IPR stimulation, cytoskeletal filaments beneath the plasma membrane were arranged in a thicker layer. Contraction of the submembranous actin cytoskeleton during exocytosis elicited by CCh/IPR may cause rearrangement of TJ strands, and modulate increased paracellular permeability.

为了阐明细胞旁液体分泌途径的形态变化，采用深蚀刻冷冻断裂(FF)复制的方法，用透射电子显微镜观察了TJ细胞骨架。用1μM的卡巴胆碱(CCH)和异丙肾上腺素(IPR)刺激离体下颌下腺。CCH/IPR刺激后，质膜下的细胞骨架丝排列较厚。CCH/IPR引起的胞吐过程中膜下肌动蛋白细胞骨架的收缩可能导致TJ链的重排，并调节细胞旁通透性的增加。

项目成果

マウス耳下腺腺房細胞でのaquaporin6 の発現

水通道蛋白6在小鼠腮腺腺泡细胞中的表达

• 发表时间: 2009
• 作者: 市川秀樹;澁川義幸;橋本貞充;田崎雅和;下野正基
• 通讯作者: 下野正基

Electrophysiological properties of AQP6 in mouse parotid acinar cells.

小鼠腮腺腺泡细胞中 AQP6 的电生理特性。

• 发表时间: 2009
• 期刊: J.Medical Investigation Proceeding 56
• 作者: Ichikawa H.;Shibukawa Y.;Sahara Y.;Tsumura M.;Qi B.;Satoh K.;Narita T.;Hashimoto S. Momose Y.;Tazaki M.;Shimono M.;Sugiya H.
• 通讯作者: Sugiya H.

Morphological evidence of paracellular transport in perfused rat submandibular glands.

• DOI: 10.2152/jmi.56.395
• 发表时间: 2009
• 期刊: The journal of medical investigation : JMI
• 作者: S. Hashimoto;M. Murakami

Presence and localization of aquaporin-6 in rat parotid acinar cells Presence and localization of aquapor in-6 in rat parotid acinar cells

大鼠腮腺腺泡细胞中水通道蛋白 6 的存在和定位 大鼠腮腺腺泡细胞中水通道蛋白 6 的存在和定位

• 发表时间: 2008
• 期刊: Cell Tissue Res. 332
• 作者: Matsuki-Fukushima M.;Has himoto S.;S imono M;Satoh K.;Fujita-Yoshigaki J;Sugiya H.
• 通讯作者: Sugiya H.

Hill AE The control of paracellular transport during salivary fluid secretion by the isolated perfused submandibular gland of rat.

Hill AE 大鼠离体灌注颌下腺在唾液分泌过程中对旁细胞运输的控制。

• 发表时间: 2009
• 作者: Murakami M;Hashimoto S;Sugiya H;Narita T;Qi B;Wei M
• 通讯作者: Wei M