Long term stability of titanimum with hydroxyapatite layer precipitated by anodic oxidation and hydrothermal treatment

通过高压水蒸气水热处理（SA处理），在阳极氧化钛表面沉积一层结晶度高、与钛基体结合力强的羟基磷灰石层，改善了化学纯钛的表面性能。报道了SA治疗的有效骨传导能力。SA处理的羟基磷灰石层在口腔环境下的稳定性在临床病例中是重要的。因此，它检查了SA处理层的长期稳定性。采用纯钛圆盘。它们在含有0.01mol/l β-甘油磷酸二钠盐五水合物和0.15mol/l醋酸钙一水合物的溶液中进行阳极氧化。施加350 V的电解电压，在阳极氧化后，用蒸馏水洗涤圆盘并干燥。水热处理在300 ℃下进行2小时。将这些样品溶解于0.9%NaCl溶液中，通过获得pH=6.0的溶液制备。 ...更多信息 乙酸和Tris-HCl。对样品进行了扫描电镜和X射线衍射分析。对第1天和第7天的样品进行SEM观察，结果表明，HA溶解后结构没有发生变化。14天后，在HA晶体内观察到溶解，但不承认所有HA晶体溶解的图像。X射线衍射分析结果表明，不存在因溶解天数和溶解时间的不同而引起的变化。将从大鼠骨髓细胞中分离的成骨细胞和破骨细胞分别培养在样品表面。培养物用Karnovsky法和四氧化锇固定，通过梯度系列乙醇脱水，并用叔丁醇冷冻干燥。在培养过程中，细胞粘附于所有标本表面，并产生大量的骨结节。浸渍样品和未浸渍样品的密度和形成速度没有差异。在形态学上，所有样本的破骨细胞无差异。表明SA处理的羟基磷灰石层对体外培养的细胞具有长期的稳定性。少

The surface property of chemically pure titanium was improved depositing a hydroxyapatite layer which exhibited high crystallinity and strong adhesion to the titanium substrate on an anodically oxidized titanium surface through the hydrothermal treatment with high pressure steam (SA treatment). The effective bone conduction ability of SA treatment was reported. The stability of SA treated hydroxyapatite layer under oral environment is important in the clinical case. Therefore, it examined a long term stability of the SA treated layer. Pure titanium discs were employed. They were anodized in a solution containing 0.01mol/l β-glycerophosphate disodium salt pentahydrate and 0.15mol/l calcium acetate monohydrate. An electrolytic voltage of 350V was applied, and after anodic oxidation the discs were washed with distilled water and dried. Hydrothermal treatment was the conducted at 300 ℃ for 2 hours. These samples were dissolved to the 0.9%NaCl solution to have prepared for pH=6.0 by gaining … More acetic acid and Tris-HCl. The samples were analyzed by SEM and X-rays diffraction analysis. The result of the observation in the SEM of the 1st and 7th day's samples, the change of the HA structure was not admitted after dissolution. After 14 days, dissolution was seen within the HA crystals, but the image that all the HA crystals are dissolving, was not admitted. As a result of X-rays diffraction analysis, the change by the difference of the dissolution number of days and solution were not admitted.Osteoblasts and osteoclasts that obtained from rat bone marrow cells, were cultured on the surface of samples. Cultures were fixed with Karnovsky' s method and osmium tetroxide, dehydrated through a graded series of ethanol, and freeze dried with t-butyl alcohol. Specimens were observed under SEM.During the culture period, the cells adhered to all samples surfaces and produced an abundant amount of bone nodules. The density and speed of formation were not different between dipping samples and non-dipping samples. Morphologically, there was no difference concerning osteoclasts for all samples. It was suggested that hydroxyapatite layer of SA treatment has long term stability for culture cells in vitro. Less