The primary calcification by irradiated osteoblasts

受辐射的成骨细胞的初级钙化

• 批准号: 13672049
• 负责人: ITOH Sozo
• 金额: $ 2.18万
• 依托单位: IWATE MEDICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672049/
• 关键词: maxillofacial prosthesis; radiation; implant; osteoblast; ストレスタンパク質

Although there have been several reports concerning the use of osseointegrated implants for maxillofacial prosthesis, little is still understood about the effects of radiation on the bone/titanium interface. Since it is quite important to know this relationship when dealing with cases where implants have been inserted into the bone after radiotherapy. We particularly concentrated on studying about the stress protein (HSP).First, we measured HSP of mouse MC3T3-E1 cells that were irradiated in vitro as a stress parameter. This study showed that the expression of HSP90a and HSP47 mRNA were not significantly enhanced by the irradiation under the dose of 40mGy, whereas it increased with the irradiation at the dose 4000mGy. There findings suggests that the low-dose irradiation probably has little effect upon the function of MC3T3-E1 cells in vitro.Next we observed the primary calcification on the pure titanium and treated titanium surface where osteoblasts were irradiated in vitro. Results showed that irradiation doses under 400mGy induced no significant changes of the primary stage of calcification on the bone/titanium and treated titanium interface. The values for 4000mGy were significantly different from control and under 400mGy concerning about the interface. The values of calcification on treated titanium were significantly different from pure titanium under 400mGy.ConclusionThe primary stage of calcification on the bone/titanium interface was affected upon the amount of radiation exposure, especially over 4000mGy and results showed the positive effects of treatment for primary calcification under 400mGy.

虽然已经有一些关于使用骨整合种植体进行颌面修复的报道，但关于辐射对骨/钛界面的影响仍然知之甚少。因为在处理放射治疗后植入物插入骨中的病例时，了解这种关系非常重要。首先，我们以体外照射的小鼠MC 3 T3-E1细胞为研究对象，测定了HSP作为应激参数的变化。结果表明，40 mGy剂量照射对HSP 90 a和HSP 47 mRNA表达无明显影响，而4000 mGy剂量照射后，HSP 90 a和HSP 47 mRNA表达增加。结果表明，低剂量辐照对体外培养的MC 3 T3-E1细胞的功能影响不大。其次，我们观察了纯钛和经辐照处理的钛表面成骨细胞的初级钙化。结果表明，400 mGy以下的辐照剂量对骨/钛和处理后的钛界面的初级钙化没有明显的影响。4000 mGy剂量组与对照组及400 mGy剂量组之间的界面损伤有显著性差异。结论骨/钛界面早期钙化受照射量的影响，尤其是4000 mGy以上，400 mGy以下的治疗对早期钙化有积极作用。