The investigation of mechanisms between PDT and metastatic effect of oral cancer

光动力疗法有望成为治疗口腔癌的一种新的激光疗法。然而，光动力治疗转移效应的机制尚不清楚。本研究旨在阐明光动力疗法的转移机制。以4-硝基喹啉N-氧化物(4NQO)诱导的F344大鼠分化型鳞癌细胞系(RSC3-E2)和未分化肿瘤细胞系(RSC3-LM)为研究对象，分别用波长为530~540 nm的光(530~540 nm)和光敏剂(血卟啉衍生物)进行光动力疗法。以小鼠成纤维细胞系为对照。以细胞凋亡指标作为判断转移影响的指标。单独给予血卟啉衍生物时，RSC3-E2和RSC3-LM细胞株均未观察到细胞凋亡。而在联合光源作用下，RSC3-E2细胞株和对照组的细胞凋亡均受到抑制。这些结果表明，在该细胞系模型中，光动力条件下可促进肿瘤的转移。

Photodynamic therapy (PDT) is expected as a new laser therapy for treating the oral cancer. However the machanisms of metastastatic effect when PDT is conducted is unknown. This study was conducted to clarify the machanisms of metastasis when PDT is conducted.Differentiated squamous cell carcinoma cell line (RSC3-E2) and undifferentiated tumor cell line (RSC3-LM) with different metastatic potential that established from a 4-nitroquinoline N-oxide (4NQO)-induced differentiated SCC in F344 rat were used for undergoing PDT using light (Wavelength 530〜540nm) and Photofrin^<TM> (hematoporphyrin derivative. Fibroblast cell line derived from mice was used as cntrol. Apoptosis indication was counted for estimating the metastatic influences. Apoptosis was not observed at RSC3-E2 and RSC3-LM cell lines when hematoporphyrin derivative was administered alone. However apoptosis was resisted at RSC3-E2 cell lines as well as control when PDT was underwent together with light source. These results suggested that metastasis would be promote on the conditions of PDT at this cell lines model.