Effect of low power laser irradiation on cultured fibroblast cells.

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The present study showed that the biological effects on the cultured human gingival cells irradiated by two different wave length low-power lasers. Ana a control was set up in the non-irradiation group.Results : Mitogen activity was increased by both wave length laser irradiations when the He-Ne watt density was 18.72 mW/cm^2, and the joule density was 5.62 J/cm^2, and when the Ga/Al/As diode watt density was 1.12 mWcm^2, and the joule density was 0.33 J/cm^2. The cell proliferation was increased under a 10% TIMP-1 and TIMP-2 free FCS+D-MEM condition. 48 hours after He-Ne irradiation and 24 hours after Ga-Al-As diode irradiation, there were significant differences. At that time, when TIMP-1 and TIMP-2 secretions in condition medium were measured with EIA,TIMP-1 secretion had significantly increased after 24 hours from irradiation with both lasers together with the He-Ne laser group and Ga-Al As diode laser group. But TIMP-2 detection was impossible by EIA.In the same culture conditions, when the Ga-Al-As diode laser was used to irradiate Gin-1 cells and the RT-PCR method was used to detect mRNA expression, an obvious difference in the TIMP-1 mRNA expression was found at 2 hours post-irradiation. But no difference in TIMP-2 mRNA expression was noted.These results suggest that low-power lasers of appropriate irradiation accelerate human gingival fibroblast cell growth, and may promote acceleration of wound healing.