The effects of three-dimensional form on the healing process of three-dimensional alveolar bone defect.

本研究的目的是阐明牙周组织再生能力的愈合过程，建立牙周组织再生能力的影响标准。制作三壁骨缺损的实验动物为6只雄性食蟹猴（Macaca fascicularis），每只猴约2 - 3岁，体重3.8 - 5.7 kg。将猴饲养在恒温恒湿条件（28 ± 1℃，50%至60%）下的单独金属笼中，并提供充足的固体猴饲料和自来水。在全身麻醉下每周进行一次口腔卫生程序，以保持牙周组织尽可能健康的状态。试验部位为左右下颌第二前磨牙和第一磨牙，在2%利多卡因局麻下拔除左右下颌第二前磨牙和第一磨牙，含肾上腺素止血。术后3个月，采用垂直松解切口的全厚粘骨膜瓣，在牙周制作不同大小的人工骨缺损，对照组在牙根面刮除后关闭瓣。Boneject组植入人工骨材料（Boneject^[圈R]，Koken Co.Ltd.，日本），并关闭皮瓣。膜组使用可吸收膜（Resolut^[圈R]，日本Gore-tex，日本）。术后3个月处死动物，制作组织切片。结果显示，所有组的2 × 1 × 1骨缺损均被骨组织完全填充。对照组2x2 x2或2x2 x4骨缺损均无骨性愈合。结果提示，对于8mm^3以上的小骨缺损，牙周组织再生治疗是有效的。

The Purpose of this study was to clarify the healing process and establish the standard of the effect of periodontal tissue re-generation ability. The experimental animals for make 3-wall bone defects were six male crab-eating monkeys (Macaca fascicularis), each monkeys were approximately 2 to 3 years of age and weighting 3.8 to 5.7 kg. The monkeys were kept in separate metal cages under constant temperature and humidistat conditions (28 ± 1℃, 50% to 60%) with sufficient solid monkey dietand tap of water. The oral hygiene procedures were performed once a week under general anesthesia to maintain the periodontal tissues in as healthy condition as possible. The test sites were the second premolar and first molar teeth in the right and left mandible.The second premolar and the first molar in the right and left mandible were extracted under local anesthesia with 2% lidocine, including epinephrine for hemostasis. Three months after, full-thickness mucoperiosteai flap with vertical releaseing incisions were raised, and made various size of artificial bone defect surrounding teeth.Control group were scaled the surface of the root and close the flap. Boneject group were implant artificial bone material (Boneject^【encircled R】, Koken Co.Ltd., Japan) and close the flap. Membrane group were used resorbable membrane (Resolut^【encircled R】, Japan Gore-tex, Japan). Three month after surgery, animals were sacrificed and made tissue section. In the results, 2x1x1 bone defect were fully filled by bone tissue in all groups. In contrast, 2x2x2 or 2x2x4 bone defects, they were no bony healing in the control group. In these results suggest that, they are no effect on the small bony defect but more than 8mm^3 of bony defect, some regenerative therapy will be usefull to regenerate periodontal tissue.