Study on Escherichia coli O-157-induced renal damage

内毒素(LPS)和维罗毒素-2(VT2)参与了大肠杆菌O-157诱导的肾损伤。然而，在注射了每种药物后，病理生理特征并没有得到充分的评估。进行这项研究就是为了解决这一问题。内毒素对大鼠的时序毒性Wistar大鼠于9：00或21：00静脉注射内毒素(5 mg/kg)，观察脏器损伤情况。21：00审判的器官损害程度明显大于9：00审判。在21：00的试验中，血清中炎性细胞因子IL-6的升高幅度更大，器官中IL-6的合成也更强。这些数据表明，内毒素的毒性依赖于其给药时间，可能是通过对IL-6反应的时间依赖性差异。抗中性粒细胞抗体对VT_2所致小鼠脏器损伤的预防作用：于注射VT_2后0小时和24小时分别给C57BL/6小鼠注射VT_2(100 Ng)和联合应用抗中性粒细胞抗体。单独注射VT_2后，血浆中性粒细胞显著升高，5天内全部死亡。使用抗中性粒细胞抗体的小鼠死亡率显著降低。提示中性粒细胞在VT_2诱导的脏器损伤中起一定作用。

Lipopolysaccharide (LPS) and verotoxin-2 (VT2) are involved in the Escherichia coli O-157-induced renal damage. However, pathophysiologic profiles are not fully evaluated after an injection of each agent. This study was undertaken to address this issue.1. Chronotoxicity of LPS in ratsLPS (5 mg/kg) was injected intravenously to Wistar rats at 9:00 or 21:00, and organ damages were evaluated. The degree of organ damages in the 21:00 trial were significantly greater than those in the 9:00 trial. The elevation in serum interleukin-6 (IL-6), an inflammatory cytokine, was greater and its synthesis in organs was more enhanced in the 21:00 trial. These data indicate that the toxicity of LPS depends on its dosing time, probably through the time-dependent difference in the IL-6 response.2. Preventive effect of anti-neutrophil antibody against the VT_2-induced organ damage in mice.VT_2 (100 ng) was injected intraperitoneally to C57BL/6 mice with and without the co-administration of anti-neutrophil mouse antibody at 0 and 24 hours following VT_2. After the injection of VT_2 alone, plasma neutrophil remarkably elevated and all animals died within 5 days after the VT_2 injection. Death rate was significantly reduced in mice with the anti-neutrophil antibody. These data suggest that neutrophil plays some role in the VT_2-induced organ damage.