Preparation of functional interface and its in situ measurement

为了开展这项国际合作工作，我们首先在西奈山医学院和东京首都大学研究生院工学研究科应用化学部门之间签订了研究协议。协议期限应为2年，与本研究相同。协议的续签将在期末讨论。在1999年，通过电子邮件讨论完成了合作工作。1999年11月，在Mt.西奈大学讨论探测生物表面的分析方法。在2000年，讨论主要通过电子邮件进行。西奈半岛约1个月。他用钛/蓝宝石激光器进行了全内反射几何结构的荧光研究实验。在本研究的预算年度结束时，在东京首都大学举行了最后一次会议， ...更多信息 Ross.与此同时，我们在大学之间达成了一项新的研究协议。我们开发了一种利用聚合物微通道制备功能表面的方法。该方法的优点在于：1）利用聚酯的原位聚合，利用一种新型的玻璃模板，研制出了可重复的聚合物微通道。2）通过在不饱和聚酯中引入分子内带有双键的功能分子，使分子功能很容易地嵌入到微通道中。3)我们还开发了一种通过微通道内壁的进一步衍生化来制备功能表面的技术。关于实际结果，请参阅发表在学术期刊上的论文。在此基础上，我们发展了一种新型的全内反射热透镜光谱法。该方法能够精确分析固液界面。我们将此方法应用于玻璃/液体界面，以探测溶解在液相中的有机染料。首先明确了染料的吸附行为、吸附速度和吸附机理。然后我们将这种方法应用到液/液界面，以了解复合物的形成机理。关于荧光研究，我们完成了新的仪器与钛/蓝宝石激光。然后应用于时间分辨荧光研究，探讨了组织因子和Anexine的状态。少

In order to proceed this international collaborative work, first we concluded an research agreement between Mount Sinai school of medicine and department of Applied Chemistry, Graduate school of engineering of Tokyo metropolitan university. The period of the agreement should be 2 years that was the same period as this study. And the renewal of the agreement will be discussed at the end of the period. At the year 1999, collaborative works were done through the discussion by E-mail. In November 1999, a meeting was held at Mt. Sinai University discussing the analytical method probing biological surface. At the year 2000, discussions are mainly made through E-mail In September 2000, Dr. Shimosaka, one of the researcher of this study, stayed at Mt. Sinai for about 1 month. He made an experiment for Fluorometric study with total internal reflection geometry by Ti/Saphire laser. At the end of the budget year of this study, final meeting was held at Tokyo Metropolitan University joining Prof. … More Ross. At the same time we concluded a new research agreement between the universities. We have developed a preparation method for functional surface utilizing polymer micro channel. The merits of the method are as follows:1) We have developed reproducible polymer micro channel using a novel glass template with in situ polymerization of polyester 2) Molecular function was easily incorporated into the micro channel by the addition ot functional molecule with double bond inside the molecule to the unsaturated polyester. 3) Also we have developed a preparation technique of functional surface by the further derivatization of inner wall of the micro channel.Regarding to the practical results, please refer to the papers published in the Academic Journals. Then we have developed a novel 'Thermal Lens spectrometry with total internal reflection geometry". The method enables precise analysis for solid-liquid interface. We apply this method to the glass/liquid interface to probe organic dye dissolved in liquid phase. We first made the adsorption behavior, adsorption velocity, and adsorption mechanism of the dye clear. Then we applied this method to liquid/liquid interface to understand the mechanism of complex formation. Regarding to the fluorometric study, we complete the novel instrument with Ti/Saphire Laser. Then applied to the experiment for time resolved fluorescence study probing the state of Anexine and Tissue factor. Less