Developmental use of novel-bioactive agents identified from Bombyx mori and wild silkmoths

在野生天蚕腺中发现了对大鼠肝癌细胞(dRLh-84细胞系)的细胞毒活性，该化学结构被确定为一种新的儿茶酚乙酸二聚体。我们一直在执行合成方法。从滞育的天牛一龄幼虫中分离到DILRG-NH_2。酰胺化五肽及其截短型ILRG-NH_2而不是LRG-NH_2和非酰胺化五肽抑制大鼠肝癌细胞的增殖。这种抑制可能是由于细胞周期停滞，而不是由于凋亡/坏死活性。从桑树硬枝插条根的乙酸乙酯提取物中分离得到一种新的异戊烯基黄酮类化合物。这种异戊烯基黄烷酮对大鼠肝癌细胞具有细胞毒活性。这些结果表明，分离的药物是任何治疗用途的候选药物。家蚕的食道下体以长…著称更多的g时间，但它的功能还没有被澄清。我们证明了这个器官产生溶菌酶，一种组织特异性的胰凝乳酶抑制物(SCI-SB)和另一种蛾已知的糖蛋白的P27K同源基因。因此，鉴定的蛋白质的性质和表达模式表明，该器官是一个与免疫相关的器官。一系列1-取代和1，5-二取代咪唑类化合物可终止天牛和舞毒蛾1龄幼虫滞育。为了了解咪唑类化合物的作用方式，我们使用合成树脂偶联的KK-42类似物，通过亲和层析分析了咪唑类化合物(KK-42)结合蛋白。从滞育个体制备的可溶性部分中分离到两个结合蛋白(KK-42bps)，大小分别为40和45 kDa。测定了45 kDa蛋白前20个残基的N末端氨基酸序列。这一结果是首次在昆虫体内发现与人工孵化有关的蛋白质。此外，我们还成功地研制了天南星丝素粉化妆水，并提出了在创伤后应激障碍的EMDR治疗中，利用昆虫声治疗PTSD的效果优于人工刺激的新技术。较少

Cytotoxic activity against rat hepatoma cells (dRLh 84 cell lines) was found in the colleterial glands of the wild wilkmoth Antheraea yamamai This chemical structure was determined as a novel dimmer of catecholacetic acid. We have been performing the synthesis method. DILRG-NH_2has been isolated from diapausing pharate first instar larvae A. yamamai. The amidated pentapeptide and its truncated version ILRG-NH_2, not LRG-NH_2 and the non-amidated pentapeptide, suppressed proliferation of the rat hepatoma cells. This suppression may be due to a cell cycle arrest rather than to an apoptotic/necrotic activity. A novel prenylated flavanone was isolated from ethyl acetate extracts of the hard-wood cutting root of Kinuyutaka, a culture of Morus alba. This prenylated flavanone exhibited cytotoxic activity against rat hepatoma cells. These results indicate that the isolated agents are any candidate for therapeutic use. The subesophageal body of the silkworm, Bombyx mori has been known for a lon … More g time, but its function has not been clarified. We demonstrated that this organ produces lysozyme, a tissue-specific chymotrypsin inhibitor (SCI-SB) and a P27K ortholog of a glycoprotein known fro another moth. As a result, the nature and expression patterns of identified proteins indicate that the organ is an immunity-associated organ. A series of 1-substituted and 1,5-disubstituted imidazoles have been shown to terminate diapause in pharate first instar larvae of A. yamamai and the gypsy moth, Lymantria dispar. To understand the mode of action for the imizadole compounds, we analyzed imidazole compound (KK-42)-binding proteins by affinity chromatography using a synthetic resin-coupled KK-42 analog. Two binding proteins (KK-42BPs) of 40 and 45kDa were isolated in soluble fractions prepared diapausing individuals. The N-terminal amino acid sequence from the first 20 residues was determined for the 45 kDa protein. This result is the first finding of a protein associated with artificial hatching in insects. In addition, we were successful in developing of makeup water of fibroin powder from A. yamamai and propose that a new technology used insect sound is better than the artificial stimulus when directed to both the right and left cerebral hemisphere in EMDR treatment for PTSD. Less