Investigation into the roles of autoantibodies in the pathogenesis of central nervous system lupus erythematosus.

我们研究了抗核糖体P蛋白抗体（抗P）的细胞表面上的人免疫活性细胞，以描绘其在中枢神经系统狼疮的发病机制中的作用的表位的存在。将来自正常健康个体的高度纯化的CD 4+和GD 8 + T细胞、单核细胞和B细胞与亲和纯化的IgG抗P反应，并用FITC缀合的F（ab '）_2、片段山羊抗人IgG染色，随后通过碘化丙啶染色进行流式细胞术分析，并对活细胞进行门控。在CD 4+和CD 8 + T细胞、单核细胞或新鲜B细胞表面未发现与核糖体P蛋白羧基端22个氨基酸序列抗原相关的表位。然而，在用固定化抗-CD 3活化后的CD 4+和CD 8 + T细胞上以及在与或不与IFN-γ孵育24小时后的单核细胞上诱导核糖体P表位的表达，而在活化的B细胞上不表达该表位。提示抗P抗体是一种抗淋巴细胞抗体，它与活化的T细胞和单核细胞反应，而不与静止的T细胞、单核细胞和B细胞反应，提示抗P抗体可能在系统性红斑狼疮免疫失调中起直接作用。狼疮性精神病患者脑脊液中抗谷氨酸受体抗体未检测到明显升高，提示抗神经元抗体识别。

We examined the presence of the epitope recognized by antiribosomal P protein antibody (anti-P) on the cell surface of human immunocompetent cells in order to delineate its role in the pathogenesis of CNS lupus. Highly purified CD4+ and GD8+ T cells, monocytes, and B cells from normal healthy individuals were reacted with affinity-purified IgG anti-P, and were stained with FITC-conjugated F(ab')_2, fragment goat anti-human IgG, followed by analysis on flow cytometry with gating for viable cells by propidium iodide staining. The presence of an epitope that is antigenically related to the earboxyl-terminal 22-amino-acid sequence of ribosomal P protein was not demonstrated on the surface offreshCD4+ and CD8+ T cells, monocytes, or fresh B cells. However, the expression of the ribosomal P epitope was induced on CD4+ and CD8+ T cells after activation with immobilized anti-CD3 and on monocytes after 24h incubation with on without IFN-γ, whereas the epitope was not expressed on activated B cells. These results indicate that anti-P is an antilymphocyte' antibody, which reacts with activated T cells and monocytes but not with resting T cells, monocytes or B cells, suggesting possible direct effects of anti-P on the immune dysregulation in systemic lupus erythematosus. We could not detect significant elevation of anti-glutamate receptor antibody in cerebrospinal fluid from patients with lupus psychosis, indicating that recognized by anti-neuronal antibody.