Analysis of the mechanism of production of pathologic autoantibodies

病理性自身抗体产生机制分析

• 批准号: 23591447
• 负责人: HIROHATA Shunsei
• 金额: $ 3.33万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591447/
• 关键词: 自己抗体; Bリンパ球; Tリンパ球; 抗CD３抗体; クラススイッチ; CTLA4; CD80; マクロファージ; 抗体産生; アバタセプト; 抗CD3抗体; CTLA-4; Staphylococcus aureus; TNF-α; IL-6

In order to delineate the mechanism of polyclonal B cell activation, we first explore the mechanism of suppression of B cell by activated T cells using the system with immobilized anti-CD3. Although it was suggested that interactions between CTLA4 on T cells and CD80/86 on B cells might involve in the suppression, we could not get direct evidence.We also examined whether IgG type autoantibodies might be produced in cultures with immobilized anti-CD3 stimulated mitomycin C-treated T cells and B cells. However, none of IgG anti-DNA, anti-Sm, anti-RNP were produced. Nor could we induce class switch of IgM anti-DNA to IgG anti-DNA with monocytes or alpha-interferon.Further studies are required to investigate the mechanism of production of IgG anti-DNA.

为了阐明多克隆B细胞活化的机制，我们首先利用固定化抗CD 3的系统探讨活化T细胞抑制B细胞的机制。虽然有人认为T细胞表面的CTLA_4和B细胞表面的CD_（80/86）之间的相互作用可能参与了这种抑制作用，但我们没有得到直接的证据。 而IgG抗DNA、抗Sm、抗RNP均未产生。 我们也不能用单核细胞或α-干扰素诱导IgM抗-DNA向IgG抗-DNA的类别转换，IgG抗-DNA的产生机制还需要进一步的研究。

项目成果

Enhanced expression of mRNA for interleukin 6 and tumor necrosis factor-alpha in CD34+ cells of the bone marrow in osteoarthritis

骨关节炎骨髓 CD34 细胞中白细胞介素 6 和肿瘤坏死因子-α mRNA 表达增强

• 发表时间: 2011
• 作者: Hirohata S;Yanagida T,Tomita T;Yoshikawa H
• 通讯作者: Yoshikawa H

Enhanced expression of flt3 mRNA and increased differentiation of immature plasmacytoid dendritic cells in CD34+ cells of the bone marrow in rheumatoid arthritis.

类风湿性关节炎骨髓 CD34+ 细胞中 flt3 mRNA 表达增强，未成熟浆细胞样树突状细胞分化增加。

• 发表时间: 2011
• 作者: Hirohata S;Tomita T;Yoshikawa H
• 通讯作者: Yoshikawa H

Challenges in Rheumatology.

风湿病学的挑战。

• 发表时间: 2011
• 作者: Fujita J;Kawaguchi M;Kokubu F;Ohara G;Ota K;Huang SK;Morishima Y;IshiiY;Satoh H;Sakamoto T;Hizawa N;Hirohata S
• 通讯作者: Hirohata S

ステロイド精神病 リウマチ専門医.

类固醇精神病风湿病学家。

• 作者: 廣畑 俊成;有沼 良幸
• 通讯作者: 有沼 良幸

Luncheon Seminar 5-2 Behcet’s disease

午餐研讨会5-2 白塞氏病

• 作者: 廣畑 俊成;有沼 良幸;廣畑 俊成;Hirohata S
• 通讯作者: Hirohata S