studies on Proliferation and Differenntiation of Fibroblast in Gastric Mucosa by HElicobacter pylori infection

为探讨肌成纤维细胞增殖的机制，用放射自显影和原位杂交的方法，对碱性成纤维细胞生长因子(BFGF)、血小板衍生生长因子(PDGF)、内皮素及其受体的结合部位进行了研究。结果发现，对照组成纤维细胞和肌成纤维细胞间充质细胞上均有bFGF、PDGF-BB和Endothelin-1的受体分布，而感染的蒙古沙土鼠感染的嗜热螺杆菌中，CS23-bFGF和耐酸的衍生型bFGF引起成肌成纤维细胞和成纤维细胞的数量显著增加。此外，由于瘦素最近引起了与脂肪储存细胞激活有关的关注，因此对瘦素及其受体的定位进行了研究。在正常蒙古沙土鼠胃粘膜中，瘦素免疫反应位于微循环单位的内皮细胞中。在幽门螺杆菌感染的生殖细胞中，Teptin及其受体在未分化的中膜细胞和肌成纤维细胞中被识别。

To clcrnfy the mechanism of the myofibroblast proliferation, the binding sites of basic fibroblast growth factor (bFGF), plactietet-derived growth factor (PDGF), endothelin as well as their receptor, ware investingated by autoradiogrophy and in sitill hybridization As a result, the recepters of bFGF, PDGF-BB and Endothelin-1 were found to be distributed on the mesenchymal cells in chiding fibroblasts and myofibroblasts in the control group, while in the Helicobacter pytori-in fected Mongolian gerbils the number of CS23-bFGF, anacid-stable bFGF derivative, brought about the marlced increase of the number of myofibroblasts and FGF receptors. In addition, the localization of leptin and its receptor was investingated because leptin has recently atracted attention in relation to activation of fat-storing cells. In the gastric mucos of the control Mongolian gevbils, leptin immunoreactivity was observed in the endothelial cells of the microcirabatory units. In Helicobacter phylori-infected gerbits, teptin and its receptor ware recognized in the undifferentiated mesonohymal cells and myofibroblasts.