The role CO radicals in the pathogenesis of shock and organ dysfunction

在目前的研究中，碳单氧化物基（CO ·）在循环性抑郁和多器官紊乱的病理学中的作用得到了调查。用300克的Wistar大鼠体内重量被去除，用65毫克/千克的五溴钠。“Trachea被诱导成为一个容易控制的呼吸。”动物被诱导到休克和多种器官紊乱，由LPS的内周注射（10毫克/千克）或通过肢体的表现引起。血液压力被监测和丰富的血液被分析为集中的硝基血红蛋白（NO-Hb）和羧基血红蛋白（CO-Hb）。ESR技术已被应用于确定NO-Hb，如Sakamoto。CO-Hb使用血气分析仪测量（Radiometer Co Ltd. ABL 625型）。动物在LPS注射组内6小时进入休克，在Caecal Ligation组内12小时进入休克。对L-canabanine进行20毫克/千克的预处理，是iNOS的抑制剂，在血液压力下保留了较高的价值，而不是羞耻操作的控制。锌Protopolphyline的管理在休克模型中的循环功能中取得了良好的结果。两个冲击模型中的NO-Hb和/或CO-Hb的血液水平揭示了一个较低的值与sham操作的控制进行比较。我们还观察到了具有两种冲击类型的单倍体细胞中HO-1的信使RNA的递减表示。

In the present study, the role of carbon monoxide radicals (CO ・) in the pathogenesis of circulatory depression and multiple organ dysfunction was investigated. Wister strain rats with 300g of body weight were anesthetized with 65 mg/kg of pentobarbital sodium. The trachea was intubated to facilitate controlled respiration. The animals were induced into shock and multiple organ dysfunction by the intra-peritoneal injection of LPS (10mg/kg) or by the ligation-perforation of cecum. The blood pressures were monitored and venous blood was taken to analyze concentrations of nitrosyl hemoglobin (NO-Hb) and carboxyhemoglobin (CO-Hb). The ESR technique was applied to determine NO-Hb according to Sakamoto. The CO-Hb was measure using blood gas analyzer (Radiometer Co Ltd. Type ABL 625).The animals fell into shock within 6 hours in LPS injected group and within 12 hours in caecal ligation group. The pretreatment of 20 mg/kg of L-canabanine, an inhibitor of iNOS, maintained high values in blood pressure than sham-operated controls. The administration of zinc protoporphylline gave forth a good result in circulatory function in shock model. The blood levels of NO-Hb and/or CO-Hb in two shock models revealed a lowered value in comparison with sham operated controls. We also observed a declined presentation of messenger RNA of HO-1 in the hepatic cells with two types of shock.