Biological activity of head and neck cancer cell lines with regard to IL-6 and MMP

我们建立了NKO-1，一种SCC细胞系，可产生高水平的G-CSF和IL-6，来自于一位低分化的上颌SCC患者的转移淋巴结。我们研究了G-CSF和IL-6对NKO-1体外生长的影响，rhG-CSF正常增强的中和G-CSF对NKO-1体外生长的影响，并进行了侵袭实验。高浓度的G-CSF能促进NKO-1细胞的体外生长，而任何浓度的hIL-6都不能促进NKO-1细胞的体外生长。G-CSF抗体对NKO-1细胞生长的影响呈剂量依赖性降低。与其他低水平分泌G-CSF和IL-6的细胞系相比，NKO-1细胞表现出较强的侵袭性。头颈癌细胞系癌细胞具有IL-6受体（RT-PCR）。这些IL-6受体的水平被IL-6受体抗体以剂量依赖性的方式降低。IL-6可降低头颈部癌细胞系的体外生长速率。我们研究了IL-6在细胞外基质膜系统中对头颈部癌细胞的侵袭性。白细胞介素-6可增强细胞的侵袭程度。此外，G-CSF增强了产生大量G-CSF的肿瘤细胞的侵袭性。在一些需要强效基因表达的应用中，如抗肿瘤应用，转基因表达效率低是一个问题。我们证明了携带HSVtk的AAV载体在与γ射线照射联合使用时能够更有效地杀死癌细胞。γ射线增强raav介导的转基因表达。

We established NKO-1, a SCC cell line that produces high levels of G-CSF and IL-6, from a metastatic lymph node obtained from a patient with a poorly differentiated maxillary SCC. We studied the in vitro effect of G-CSF and IL-6 on the growth of NKO-1, the in vitro effect of neutralizing G-CSF on the growth of NKO-1 normally enhanced by rhG-CSF, and invasion assay. The in vitro growth of NKO-1 cells was enhanced by high concentrations of G-CSF, but not by any concentration of hIL-6. The effect of G-CSF on NKO-1 cell growth was reduced by antibody to G-CSF in a dose-dependent manner. Compared with other cell lines, which produce low levels of G-CSF and IL-6, NKO-1 cells showed strong invasiveness.Cancer cells of head and neck cancer cell lines have IL-6 receptors (RT-PCR). The levels of those IL-6 receptors were reduced by antibody to IL-6 receptors in a dose-dependent manner. The in vitro growth rates of cancer cells of head and neck cancer cells lines were reduced by IL-6. We studied the invasiveness of cancer cells of head and neck cancer cell lines with IL-6 in an extracellular matrix membrane system. The degree of invasiveness was enhanced by incubating cells with IL-6. In addition G-CSF enhanced the invasiveness of tumor cells that produced large amounts of G-CSF.Low efficiency of transgene expression can be a problem in some applications that require potent gene expression such as antitumor applications. We demonstrate that AAV vector harbouring the HSVtk is able to kill cancer cells more efficiently when used in combination with γ-ray irradiation. γ-rays enhance rAAV-mediated transegene expression.