Immobilization of cell adhesive protein on dental implant

本研究的目的是将纤维连接蛋白直接结合在三酰氯（2,2,2 -三氟乙烷磺酰氯）处理过的钛表面，以促进种植牙与牙龈和/或上皮组织的紧密连接。镜面抛光后的钛基端羟基与三酰氯在37℃下反应2天。Tresylated钛盘在纤维连接蛋白/PBS溶液中浸泡24 h，温度37℃。通过x射线光电子能谱（XPS）测量中的F1s和O1s光谱以及傅里叶变换红外反射吸收光谱仪（FT-IR-Ras）测量中的-O-S-O_2键，证实了钛与三酰氯的碱性OH活化反应。纤维连接蛋白与钛反应后的XPS光谱表明，三酰氯处理对末端OH基团的活化效果显著。在三烷基化钛上氩离子刻蚀60 s后，附着的纤维连接蛋白产生的N1s峰仍然存在。而未经三酰氯处理的样品，经过10 s氩离子刻蚀后，N1s峰几乎消失。超声清洗60 s后，纤维连接蛋白与三烷基化钛的直接附着未被去除。纤维连接蛋白是一种众所周知的细胞粘附蛋白，利用三酰氯活化技术可以很容易地附着在钛表面。

The aim of this study is to bound fibronectin directly on tresyl chloride (2, 2, 2 -trifluoroethanesulfonyl chloride) treated titanium surface for the development of tight connection of dental implant to gingival and/or epithelial tissue. Basic terminal OH groups of mirror polished titanium were reacted with tresyl chloride at 37 ℃ for 2 days. Tresylated titanium disk was immersed into the fibronectin/PBS solution for 24 h at 37 ℃.The activation reaction of basic OH of titanium with tresyl chloride was confirmed by F1s and O1s spectra in the measurement of X-ray photoelectron spectroscopy (XPS) and -O-S-O_2 bonds in the measurement of the Fourier transform infrared Reflection-Absorption spectrometer (FT-IR-Ras). XPS spectra after the reaction of fibronectin with titanium demonstrated the remarkable effect of the activation of terminal OH groups with tresyl chloride treatment. N1s peak derived from attached fibronectin was still remaining after 60 s argon-ion etching on the tresylated titanium. On the contrary N1s peak of the specimen without tresyl chloride treatment was almost disappeared after only 10 s argon-ion etching. Direct attachment of fibronectin to tresylated titanium was not removed after 60 s ultrasonic cleaning.Fibronectin, well-known cell-adhesive proteins, could be easily attached on thetitanium surface using tresyl chloride activation technique.