Basic research for applying human lactoferrin to periodontal treatments

1.测定了15例牙周沟液和7例唾液中人乳铁蛋白的含量，结果表明：1.牙周沟液和唾液中的人乳铁蛋白水平均显著高于唾液(平均值分别为226μg/ml和20μg/ml)。HLF在牙周病牙根上的分布经兔抗HLF抗体免疫荧光染色后，荧光显示区与结石区一致。从提取的根中提取粉状样品，采用配备圆形毛刺的电机驱动系统。收集标本并按位置分为三组：I、II、III层(从根面到髓腔)。与未发现结石的I层相比，有结石的I层的HLF水平显著升高。HLF和内毒素水平从第I层到第III层逐渐降低。我们测量了19名受试者的龈上菌斑和龈下菌斑中的HLF值，结果显示，牙菌斑中的HLF值没有显著差异，但两者之间存在显著的相关性(0.609，p&lt；0.01)。对伴放线放线杆菌感染的THP-1细胞的影响加入HLF后，THP-1细胞的乳酸脱氢酶活力、DNA片段化程度和肿瘤坏死因子-α的产生均降低。这些发现表明，中性粒细胞释放的HLF与内毒素结合，抑制了内毒素对宿主细胞的免疫生物学效应，从而达到宿主保护的目的。

1. Human lactoferrin (hLf) in gingival crevicular fluid (GCF) and salivaWe measured hLf levels in 15 GCF samples and 7 saliva samples, showing that hLf levels in GCF were significantly higher than in saliva (mean hLf levels; 226 μg/ml in GCF and 20μg/ml in saliva).2. Distribution of hLf on periodontally-diseased dental rootThe area displaying fluorescence was in agreement with the area with calculus after immunofluorescence staining with rabbit anti-hLf antibody. Powdery samples were prepared from extracted roots with a round bur-equipped electric motor-driven system. The samples were collected and partitioned into three groups on the basis of location: Layers I, II and III (from the root surface to the pulp cavity). Significantly higher hLf levels were detected in Layer I from the area with calculus in comparison to Layer I from the area without calculus. hLf and endotoxin levels decreased from Layer I to Layer III.3. hLf levels in supra- and subgingival dental plaqueWe measured hLf levels in supra- and subgingival dental plaque from 19 subjects, showing that hLf levels in supra- and subgingival dental plaque were not significantly different, while the levels were significantly correlated (0.609, p<0.01).4. Effect of hLf on A. actinomycetemcomitans-infected THP-1 cellsLDH activity, DNA fragmentation and TNF-α production in the infected THP-1 cells were reduced upon addition of hLf. These findings suggested that hLf released by neutrophils is bound to LPS leading to inhibition of the immunobiological effects of LPS to host cells, which results in host protection.